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Probing Proton Translocation in Influenza A/M2 Proteoliposomes - A systematic Approach to Membrane-Protein Reconstitutions

Popular Abstract in Swedish Allt levande består av celler. Cellen är livets allra minsta byggsten. Cellen har ett hölje som kallas cellmembran. Detta membran skiljer cellens inre som kallas cytosol från omvärlden. I detta cellmembran sitter olika protein inbäddade. Genom dessa protein kommunicerar och utbyter cellen ämnen med sin omgivning. Många av dessa protein är mål för läkemedel och är därförAn improved method for reconstituting membrane proteins into artificial liposomes for quantitative functional analysis is presented. A number of key parameters for reconstitution by detergent removal are assessed in this thesis: The lipid-to-protein ratio, the detergent-to-lipid ratio and the lipid and cholesterol composition. New porphyrin-based pH-probes are evaluated. Based on this systematic,

ω-Transaminase Catalyzed Synthesis of Chiral Amines - Process Improvements Through Whole-cell Immobilization and in situ Product Removal

Popular Abstract in Swedish Populärvetenskaplig sammanfattning Den här avhandlingen handlar om förbättringar aven process för att producera sk. kirala aminer -en typ av molekyler som ingår bl.a. i många läkemedel. Kirala molekyler är de som finns i två former, sk. enantiomerer, som är varandras spegelbild. Trots sina kemiska och fYsikaliska likheter kan de ha mycket olika biologisk effekt. Ett välThe industrial use of biocatalysts is increasingly important in the production of e.g. pharmaceuticals, chemicals, fuels and foodstuffs. A successful biocatalytic process is dependent on many factors, such as the biocatalyst properties, the nature of the reaction, reactor type and downstream processing. This work is focused on two important issues: Immobilization of the biocatalyst and in situ pro

Dissipation in Frequency Selective Structures

Dissipation in Frequency Selective Structures (FSS) of aperture/slot type is studied. The FSS consists of an arbitrary number of aperture layers and dielectric layers. An aperture layer consists of a conducting plate with a periodic array of apertures, with arbitrary cross-section. The dissipation in an FSS is due to losses in the dielectric material, and losses due to finite conductivity in the me

Streamlining a Transplantation Survival Prediction Program with a RDF Triplestore

In this paper, we describe the conversion of a heart trans- plantation dataset in the RDF format and its application in a survival prediction program. The International Society for Heart & Lung Trans- plantation (ISHLT) maintains a registry of heart transplantations that it gathers from operations performed worldwide. The US United Net- work for Organ Sharing (UNOS) and the Scandinavian Scandi

Fabricated Selves: apparel and spatio-temporal boundaries

Broadening conventional sociological concerns, this paper establishes a social psychological account of queer theory, and an associated understanding of subject formation, in order to examine the relationship between the continuously emerging self and the dressed body by taking its departure in the concept and phenomenon of cross-dressing. Focusing the interrelationship between bodily gestures and

From data to decision - learning by probabilistic risk analysis of biological invasions

Predicting an uncertain future with uncertain knowledge is a challenge. The success of efforts to preserve biodiversity, to maintain biosecurity and to reduce a negative impact from climate change, depend on scientifically based predictions of future events. The ongoing introduction of non-indigenous species threatens ecological systems for which empirical data is sparse and scientific knowledge i