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Investigation of limiting metabolic steps in the utilization of xylose by recombinant Saccharomyces cerevisiae using metabolic engineering

A Saccharomyces cerevisiae screening strain was designed by combining multiple genetic modifications known to improve xylose utilization with the primary objective of enhancing xylose growth and fermentation in xylose isomerase (XI)-expressing strains. Strain TMB 3045 was obtained by expressing the XI gene from Thermus thermophilus in a strain in which the GRE3 gene coding for aldose reductase was

Immunohistochemical localization of calcitonin receptor-like receptor and receptor activity-modifying proteins in the human cerebral vasculature

Calcitonin gene-related peptide and adrenomedullin belong to a structurally related neuropeptide family and are potent vasodilators expressed in the trigeminovascular system. The molecular identity of receptors for these proteins has only recently been elucidated. Central to functional binding of these neuropeptides is the G-protein-coupled receptor, the calcitonin receptor-like receptor (CRLR), W

Visualization of micropatterned complex biosensor sensing chemistries by means of scanning electrochemical microscopy

Redox hydrogel-based micropatterned complex biosensor architectures, used as sensing chemistries in amperometric ethanol or glucose biosensors, were deposited on gold, graphite or glass. Well-localized immobilization of active hydrogels with variable compositions was achieved by dispensing 100 pl droplets of cocktails containing alcohol or glucose dehydrogenase, redox polymer (PVI(13)dmeOs) and cr

Formoterol inhaled as dry powder or via pressurized metered-dose inhaler in a cumulative dose-response study

Formoterol administered by a dry-powder (DP) capsule inhaler was compared with a pressurized metered-dose inhaler (pMDI) with regard to bronchodilating and systemic effects. The study used a double-blind, crossover, double-dummy technique. Twelve patients with moderate reversible asthma in a stable phase were examined on two separate study days, and the inhalers were given in randomized order. Aft

Wnt-5a signaling in breast cancer metastasis

Breast cancer is the most common form of malignancy affecting women in the Western world. Today, anticancer treatment can control primary breast tumors, but treatment and prevention of metastatic disease still represents a major challenge in the management of breast cancer patients. It has been shown that Wnt-5a plays an important role in breast cancer, since loss of that protein is associated wit

An 8-GHz beamforming transmitter IC in 130-nm CMOS

An 8-GHz beamforming transmitter IC has been designed in a 130-nm CMOS process. Two power amplifiers with independently controllable phase enable the beamforming. The phases are digitally controllable over the full 360° range, which is accomplished by binary weighting of quadrature phase signals in the power amplifiers. The quadrature phase signals are generated by a quadrature voltage controlled

Photoelectron escape depth in Bi2Sr2CaCu2O8+x

Photoelectron spectra of Sr and Ca shallow core levels in Bi2Sr2CaCu2O81, (Bi2212) single crystals exhibit a strong attenuation effect because the photoelectron escape depth is very short in this material. This results may have a strong impact on the interpretation of ARPES and STM spectra near the Fermi level since it suggests the electronic states of planes other than CuO play a role in both tun

Modeling Fire Growth on Combustible Lining Materials in Enclosures

An extensive research program, dealing with fire growth on combustible wall lining materials, has been ongoing in Sweden over the last decade. Several lining materials were tested in bench-scale fire tests in order to derive basic material flammability parameters. The same materials were also tested in a full scale room test and a 1/3 scale room test for two different scenarios, A and B. Scenario

Regional tissue metabolism during open or endovascular abdominal aortic aneurysm surgery

Objective: to investigate differences in regional tissue metabolism between open and endovascular aneurysm repair utilising a microdialysis technique. Materials: eight patients receiving endovascular stent grafts (STENT) and six patients undergoing open aortic surgery (OPEN) for abdominal aortic aneurysms were studied prospectively but non-randomised. Method: microdialysis catheters placed subcuta

Multiple Affiliations: Autobiographical Narratives of Displacement by (Im)migrant U.S, Women

Multiple Affiliations explores the autobiographical negotiations of memory and multilocality articulated by five (im)migrant women writers writing from, and being read (primarily) within the US. Texts as diverse as Korean-American Theresa Hak Kyung Cha's Dictée (1982), Polish (Jewish)-American Eva Hoffman's Lost in Translation: Life in a New Language (1989), Chinese-American Maxine Hong Kingston's

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Popular Abstract in Swedish Reklamen är ett oundvikligt inslag i vår vardagsmiljö och en viktig del av vår dagliga massmediekonsumtion. I den här studien har jag använt mig av tre italienskspråkiga reklambudskap för olivolja (Carapelli), pasta (Voiello) och kaffe (Lavazza) . I syfte att kunna belysa vilken viktig roll mottagarens sociokulturella bakgrund och encyklopediska kunskapsnivå spelar för The aim of this study is to describe how Italian advertising for olive oil, pasta and coffee reflects traditions, customs and culturally specific patterns regarding the ways in which these products are used in Italy, and how these culturally specific patterns are interpreted by native readers and non-native readers (Swedish students). The study is based on 22 Italian and 22 Swedish interpretations

CHOP versus MACOP-B in aggressive lymphoma--a Nordic Lymphoma Group randomised trial

BACKGROUND: The long-term survival of patients with advanced stage aggressive lymphoma has not improved significantly during the last twenty years. In a randomised trial, the efficacy of MACOP-B, a six-drug weekly chemotherapy regimen, was compared to CHOP, the current standard regimen, in terms of overall and failure-free survival, toxicity and health related quality of life. PATIENTS AND METHODS

GB virus C/hepatitis G virus infection in patients investigated for chronic liver disease and in the general population in southern Sweden

Serum samples from patients referred for liver biopsy for investigation of suspected chronic liver disease (n = 286) and from healthy middle-aged volunteers (n = 445) were analyzed for markers of exposure to GB virus C/hepatitis G virus (GBV-C/HGV), hepatitis B virus and hepatitis C virus. GBV-C/HGV analyses included GBV-C/HGV PCR for detection of viremia and GBV-C/HGV enzyme-linked immunosorbent