The aminoterminal portion of cerebrospinal fluid cystatin C in hereditary cystatin C amyloid angiopathy is not truncated. Direct sequence analysis from agarose gel electropherograms
The isolated amyloid substance in hereditary cystatin C amyloid angiopathy (HCCAA) is mainly composed of a cystatin C variant devoid of the 10 amino terminal amino acid residues of extracellular cystatin C from healthy individuals. We have developed a procedure for protein sequencing directly from agarose gel electropherograms and used this in conjunction with isoelectric focusing to investigate t