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Primordial Capsid and Spooled ssDNA Genome Structures Unravel Ancestral Events of Eukaryotic Viruses
Marine algae viruses are important for controlling microorganism communities in the marine ecosystem and played fundamental roles during the early events of viral evolution. Here, we have focused on one major group of marine algae viruses, the single-stranded DNA (ssDNA) viruses from the Bacilladnaviridae family. We present the capsid structure of the bacilladnavirus Chaetoceros tenuissimus DNA vi
Capsid structure of a marine algal virus of the order picornavirales
The order Picornavirales includes viruses that infect different kinds of eukaryotes and that share similar properties. The capsid proteins (CPs) of viruses in the order that infect unicellular organisms, such as algae, presumably possess certain characteristics that have changed little over the course of evolution, and thus these viruses may resemble the Picornavirales ancestor in some respects. H
Microstructural deformation of zircon during impact metamorphism
Impact craters are among the most pervasive geological features on solid bodies throughout the inner Solar System. The heavily cratered surface of the Moon offers a stark visual record of the Solar System’s early history of intense bombardment, a record largely absents from Earth’s dynamic surface. Despite having experienced a comparable flux of impact events over deep time, Earth's geological pro
The legacy of names : Persistence in social status in Sweden 1865-2015
This study explores how social origin, reflected by occupation and family name from both parents, has been passed down over the past 150 years in Sweden. It finds that intergenerational rank-rank associations in occupational status – a typical measure of social mobility – have remained surprisingly constant at levels around 0.27 – as in the US (Song et al., 2020). However, intergenerational correl
Real-time data processing for serial crystallography experiments
We report the use of streaming data interfaces to perform fully online data processing for serial crystallography experiments, without storing intermediate data on disk. The system produces Bragg reflection intensity measurements suitable for scaling and merging, with a latency of less than 1 s per frame. Our system uses the CrystFEL software in combination with the ASAP::O data framework. In a se
Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX.
The Lysinibacillus sphaericus proteins Tpp49Aa1 and Cry48Aa1 can together act as a toxin toward the mosquito Culex quinquefasciatus and have potential use in biocontrol. Given that proteins with sequence homology to the individual proteins can have activity alone against other insect species, the structure of Tpp49Aa1 was solved in order to understand this protein more fully and inform the desig
Flood analysis and protection system : Application to The Hongru River
The definition of a decision support system(DSS) may be : human-computer interactive software system to solve semi-structured decisional problems for decisional makers with data utilizing, information management, knowledge, as well as model techniques. DSS only provide supporting information about making decision for the decision maker without stipulate the decision itself. Decision is made by hum
Sea-based freshwater storage experiment
The Cerebellar Mutism Syndrome : Risk Assessment, Prevention and Treatment
Cerebellar mutism syndrome (CMS) has received increasing attention over the last decades as a complication of posterior fossa tumour surgery in children. Risk factors, aetiological aspects, and treatment measures of the syndrome have been investigated, yet the incidence of CMS remains unchanged. Overall, we are currently able to identify patients at risk, but we are unable to prevent it from occur
High-resolution comparative atomic structures of two Giardiavirus prototypes infecting G. duodenalis parasite
The Giardia lamblia virus (GLV) is a non-enveloped icosahedral dsRNA and endosymbiont virus that infects the zoonotic protozoan parasite Giardia duodenalis (syn. G. lamblia, G. intestinalis), which is a pathogen of mammals, including humans. Elucidating the transmission mechanism of GLV is crucial for gaining an in-depth understanding of the virulence of the virus in G. duodenalis. GLV belongs to
Structure of the T=13 capsid of infectious pancreatic necrosis virus (IPNV)-a salmonid birnavirus
Birnaviruses infect a broad range of vertebrate hosts, including fish and birds, and cause substantial economic losses in the fishery and livestock industries. The infectious pancreatic necrosis virus (IPNV), an aquabirnavirus, specifically infects salmonids. While structures on T=1 subviral particles of the birnaviruses, including IPNV, have been studied, structural insights into the infectious T
Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source
Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Ad
Deletion of fbiC in Streptomyces venezuelae removes autofluorescence in the excitation-emission range of cyan fluorescent protein
Autofluorescence poses an impediment to fluorescence microscopy of biological samples. In the Gram-positive, soil-dwelling bacteria of the genus Streptomyces, sources of autofluorescence have not been examined systematically to date. Here, we show that the model organism for the genus, Streptomyces venezuelae, shows autofluorescence in two of the commonly used fluorescence channels for visualizing
CMS-06THE NOPHO-EUROPEAN STUDY ON CEREBELLAR MUTISM SYNDROME (CMS)
BACKGROUND: The cerebellar mutism syndrome (CMS) is one of the most disabling late effects after neurosurgery for a posterior fossa tumour in childhood. The reported incidences vary substantially in previous studies. AIMS: Pathophysiology is unknown, but damage to cerebello-thalamo-cerebral circuits is likely. The study focuses on the risk factors for development and severity of CMS including surg
Genetics of circulating proteins in newborn babies at high risk of type 1 diabetes
Type 1 diabetes is a chronic, autoimmune disease characterized by the destruction of insulin-producing β-cells in the pancreas. Early detection can facilitate timely intervention, potentially delaying or preventing disease onset. Circulating proteins reflect dysregulated biological processes and offer insights into early disease mechanisms. Here, we construct a genome-wide pQTL map of 1985 protein
Gulf Women and Anti-European Imperialism : Forgotten Gender Discourses in Interwar Iran’s Shi’i Reformation Movement
Often treated as simply anti-modernism, a focus on three reformist figures with transnational intellectual ties—Shariat Sangelaji, Asadollah Kharaqani, and Muhammad Khalesizadeh—reveals how Iranian natives of the Gulf littoral contributed to reconstitute modern life by criticizing the global structures of power arising with European imperialism. Blurring the dichotomy of modern/traditional, Iran a
Witnessing against a divide? : An analysis of early Holocaust testimonies constructed in interviews between Jewish and non-Jewish Poles
This article makes visible and analyzes survivor testimonies gathered in interviews between Jewish and non-Jewish Polish survivors of Nazi persecution in Sweden in 1945–1946. By examining the content of the testimonies in relation to the distinct context, it contributes to research about early documentation efforts and their role in shaping Polish memory of the Holocaust as well as post-conflict r
Postoperative word-finding difficulties in children with posterior fossa tumours : a crosslinguistic European cohort study
PURPOSE: Posterior fossa tumour (PFT) surgery carries a risk of mutism or severely reduced speech. As for higher-cognitive language functions, word-finding difficulties have been reported, but no study has compared pre- and postoperative word-finding speeds to identify impairment caused by surgery. The current study investigated changes in word-finding ability associated with PFT surgery and exami
Witnessing the Suffering of Others in Watercolor and Pencil : Jadwiga Simon-Pietkiewicz’s Holocaust Art Exhibited in Sweden, 1945-46
In the Holocaust’s immediate aftermath (1945–1946), a small gallery in Lund, Sweden exhibited the paintings and drawings of Polish artist Jadwiga Simon-Pietkiewicz, which depicted her former fellow inmates in the Ravensbrück concentration camp. This exhibit and subsequent exhibitions elsewhere in Sweden marked rare instances of early postwar Holocaust art displayed in a country that had been relat