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Hydroxylamine was used to cleave the Asn-Gly peptide bond between the fusion partner and the antimicrobial peptide of interest, a magainin derivative (MSI-344). The efficiency of reaction depended on the hydroxylamine concentration, denaturant, pH, and the fused protein concentration. The optimal cleavage solution consisted of guanidine ⋅ HCl as the denaturant, pH 8.1, and 6.7 mg ml−1 of fuse

Triton X-114 and cation-exchange chromatography, SP-Sepharose FF, removed endotoxins from solutions containing recombinant histone H1.5. Dissociated endotoxins were removed but fractions containing histone H1.5 were enhanced in the elution step. The final concentration of endotoxins, measured by a limulus amoebocyte lysate (LAL) assay, was below 0.05 EU mg−1 histone H1.5. The recovery of protein w

A magainin derivative, designated MSI-344, was produced in Escherichia coli as fusion protein, by utilizing a truncated amidophsphoribosyltransferase of E. coli as a fusion partner. Bacterial cells transformed with the gene encoding the fusion protein were grown to a high cell density and induced with isopropyl-1-thio-b-D-galatoside (IPTG) to initiate product expression. The fusion protein was a

An Escherichia coli expression system has been constructed for production of biologically active recombinant histone H1.5. A process of fermentation and purification method at a large scale has been developed. Recombinant histone H1.5 was released from the high density cultured cells by high-pressure homogenization. For an efficient removal of cell debris and partial purification of basic histone

Capillary leak syndrome is a serious postoperative complication in neonates and infants after open-heart surgery for CHD. The aim of this study was to investigate the relationship between sternal opening width and the occurrence of capillary leak syndrome in patients undergoing cardiac surgery with delayed sternal closure.We retrospectively analysed the clinical data of neonates and infants (aged

Förhållandet mellan staten och det vi idag kallar det civila samhället har under de senaste århundradena radikalt ändrat karaktär när det gäller organiseringen av religionsutövning

Disjunct distribution patterns have long intrigued biogeographers, sparking ongoing debates about the mechanisms driving the current distribution of biodiversity. Among the most discussed patterns are long-distance dispersal and vicariance. While these patterns have been extensively studied in plants, marine taxa, mammals, and some invertebrates, they remain less explored in groups like moths. In

Electrodialysis is a water desalination technology that enables selective separation of ions, making it a promising solution for sustainable water reuse. The selectivity of the process is mainly determined by the properties of ion exchange membranes that can vary depending on the composition of ions in water, such as water uptake and charge density. In this work, we studied selective adsorpti

This article presents findings from a quantitative analysis of 215 case files regarding incidents of the crime of sexual molestation reported to the Swedish Police. We track the incidents through the criminal justice system and show a clearance rate of 9%. The clearance rate is higher when the attack is of a physical nature, the suspect is a stranger, and the incident occurs in a public place. Whi

In some ways, it can feel like conducting empirical research has become more and more difficult. Some standards that were widely agreed upon in the past for conducting research on gender and/or sex appear to be contested today (see Fraser, 2018). Some questions that may feel uncontroversial to ask for some research participants are a source of minority stress, exclusion, and outrage for others. Ca

The ecosystem response to disturbances is a central topic in ecology, and there is growing awareness of the impacts of human-driven climate change on ecosystem stability to disturbances. Terrestrial ecosystems are increasingly exposed to more drought and rainfall events as climate change intensifies. In soils, bacteria and fungi play a key role in ecosystem functioning, yet their responses to drou

This study investigates the incentives and barriers influencing AI ethics adoption in unregulated environments, drawing on semi‐structured interviews with AI professionals in Bangladesh. Our findings reveal that external pressures, such as international client requirements, drive companies to implement ethical measures, yielding both economic benefits and enhanced reputation. Conversely, tight dea

Detecting a single elementary charge has become an important task to achieve as applications within quantumtechnologies such as for qubit readout, nanothermodynamics or single shot photodetection rely on the locationof single electrons. The use of microwave resonators as the readout method for the charge sensitive single electrondevices, such as quantum dots, has been extensively used to achieve h

Let μ be a positive measure supported on a planar domain Ω. We consider the behavior of the balayage measure ν:=Bal(μ,∂Ω) near a point z0∈∂Ω at which Ω has an outward-pointing cusp. Assuming that the order and coefficient of tangency of the cusp are d>0 and a>0, respectively, and that dμ(z)≍|z-z0|2b-2d2z as z→z0 for some b>0 (here d2z is the Lebesgue measure on C), we obtain the leading order term

Heterogeneous integration of III-V narrow bandgap transistors on silicon technology is desirable for high frequency circuit implementations. Such high-speed transistors must, however, scale to large gate widths to be suitable for general circuit design. Averaging among many variable channels is a key challenge for nanowire devices. A simplified, but high-speed compatible, nanowire device process w

We report a Mueller matrix ellipsometer design using dual continuously rotating anisotropic meta wave plates, which determines the full set of Mueller matrix elements in the terahertz spectral range. The instrument operates in the frequency domain and employs a frequency tunable, solid state synthesizer based, continuous wave terahertz source with sub-MHz bandwidth. The implemented source permits