Using CRISPR/Cas9 to regulate enzyme expression levels in the Weimberg pathway for optimal xylose consumption in Saccharomyces cerevisiae
Saccharomyces cerevisiae is a commonly used industrial organism which cannot consume xylose naturally. This is a problem since xylose is a major component in renewable resources, for instance lignocellulose. The Weimberg pathway is a pathway that converts xylose into α-ketoglutarate. It has been successfully integrated into S. cerevisiae, enabling it to utilize xylose. The successful strain had th