A general method to directly obtain the DNA sequence of the variable regions of any immunoglobulin chain using a mixture of oligomer primers and the polymerase chain reaction (PCR) is described. Mixed oligonucleotide primers corresponding to the 5′ signal peptide and a conserved 3′ constant region primer were used for enzymatic amplification of each of the heavy and light chain variable regions of

The diagnosis of acute, non-traumatic diseases of the lower respiratory tract requires exact knowledge of the specific anatomy, physiology and pathology of the pediatric chest. The absolutely and relatively smaller airways, as compared with those of adults, and the undeveloped collateral ventilation result in radiological appearances that are unique in children. Viral pneumonia is predominant only

OBJECTIVE: [corrected] To investigate the relationship between hormone replacement therapy (HRT), smoking, and cancer incidence.METHODS: Baseline interviews were conducted from 1990 to 1992 in a population-based cohort of 29,508 Swedish women aged 25-65 years with no history of cancer. Cancer incidence in the cohort was assessed through December 31, 1999, with the Swedish Cancer Registry, the Popu

This thesis treats regression analysis when either the dependent or the independent variable is censored. We deal with quantile regression when the dependent variable is censored. Using the independence between the true values and the censoring limits the quantile function for the true values can be rewritten as another quantile function of the observed, censored values, where the quantile value i

In this thesis a number of computer vision problems is discussed, in particular we study the problem of view synthesis. The focus is on using complex features to determine the geometry of the scene and the cameras, in contrast to traditional point-based methods. The thesis is divided into two parts: In the fist part geometric relations between different features, corresponding in several images

The role of various material fire properties in the model of fire growth developed by Magnusson and Karlsson [l] is examined in the context of the dimensionless variables containing them. It is shown that their successful representation of their calculations by power law (one for fires which do not flashover and one for those which do) permits one to devise weighting factors for the rate of heat r

Olanzapine (Zyprexa(R)) was approved for general prescription in Sweden in November 1996, and an HPLC-based therapeutic drug monitoring (TDM) routine for serum olanzapine (OLA) and its major metabolite. N-demethylolanzapine (DMO) was established in February 1997. During 1997 to 1999, a total of 753 TDM requests for a total of 545 Swedish patients was analyi.ed. Additional patient information oil c

A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responde

The polycations spermine, neomycin and polylysine potentiated Ca(2+)-activated force in beta-escin permeabilized guinea-pig ileum strips. The effect was inhibited by the calmodulin antagonists trifluoperazine, mastoparan and W13. Potentiation was slow or absent in chi-toxin permeabilized strips, indicating dependence on penetration of the polycations into cells. The effects of spermine and neomyci