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This study was conducted to investigate Babesia canis vogeli, B. canis canis, B. canis rossi, B. gibsoni, Hepatozoon canis, Ehrlichia canis and Anaplasma phagocytophilum species, and to determine the molecular characterizations of the isolates in totally 400 whole blood samples of dogs in Kayseri region. According to the Real Time PCR results, the prevalence of E.canis, B.canis canis, B. gibsoni,