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As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in de

Polymer electrolyte membrane fuel cell (PEMFC) stacks are widely studied for their own advantages. The flow maldistribution in unit cells may severely influence the fuel cell stack performance, mainly including the uniformity of current density and the voltage. Investigations of flow maldistribution in PEMFC stacks are rarely found, and presented results are unsystematic, scattered, and even contr

The characteristic off periods that develop over time in subjects with Parkinson's disease (PD) on chronic levodopa therapy are usually considered to be motor complications but more recently the important contribution of non-motor off and non-motor fluctuations has also been acknowledged. Early-morning off (EMO) periods in PD patients are known to be a cause of significant disability, in addition

We report a simple, handy and affordable optical approach for precise number-of-layers determination of graphene on SiC based on monitoring the power of the laser beam reflected from the sample (reflectance mapping) in a slightly modified micro-Raman setup. Reflectance mapping is compatible with simultaneous Raman mapping. We find experimentally that the reflectance of graphene on SiC normalized t

Two subspecies of Helianthemum nummularium (L.) Mill. occur in southern Scandinavia. The key character distinguishing the two subspecies is a dense cover of stellate hairs on the abaxial surface of the leaves in subsp. nummularium that are not found in subsp. obscurum. Stellate hairs on the abaxial surface of the leaves are not a discrete character, but occur in a more or less continuous range fro

Real-time applications introduce new requirements on wireless networks and impose quality thresholds on parameters like delay, jitter, throughput, and packet loss in order to run smoothly. This paper addresses this issue by presenting a MAC scheme that offers real-time applications the opportunity to reserve transmission time based on their QoS requirements for contention-free medium access. Our s

There is strong circumstantial evidence that certain heavy, unstable atomic nuclei are 'octupole deformed', that is, distorted into a pear shape. This contrasts with the more prevalent rugby-ball shape of nuclei with reflection-symmetric, quadrupole deformations. The elusive octupole deformed nuclei are of importance for nuclear structure theory, and also in searches for physics beyond the standar

This article examines the way that Uruguayan author Mauricio Rosencof reconstructs family genealogy and self identity in Las cartas que no Ilegaron. The materiality of memory has an important place in the novel: the superimposed stories take shape through spaces and objects tied to the protagonist's childhood and the family history. The author employs a double entendre in which referential immedia

Immobilizing biomolecules with retained functionality and stability on solid supports is crucial for generation of sensitive immunoassays. However, upon use of conventional immobilization strategies, a major portion of the biomolecules (e.g. antibodies) frequently tends to lose their bioactivity. In this study, we describe a procedure to immobilize human single-chain variable fragment (scFv) via g

Considerations of the backscattering data for the Schrodinger operator H-v = -Delta + v in R-n, where n >= 3 is odd, give rise to an entire analytic mapping from C-0(infinity)(R-n) to C-0(infinity)(R-n), the backscattering transformation. The aim of this paper is to give formulas for B-2(v, w) where B-2 is the symmetric bilinear operator that corresponds to the quadratic part of the backscattering

Hemicelluloses extracted from wheat bran can be concentrated and purified by ultrafiltration. However, the viscosity of the process solution is rather high (about 5 mPa s at 80 C and a shear rate of 100 s1), which results in a relatively low flux. The solution was subjected to heat pretreatment in an attempt to decrease the viscosity and increase the flux. Pretreatment of the solution at 80 C for

A novel molecularly imprinted polymer (MIP) powder designed for the selective removal of interfering chlorophyll pigment from heavily pigmented green plant extracts during pesticide residue analysis or analysis for bioactives in natural product research is reported. The polymer powder imprinted with chlorophyll.. was synthesized by copolymerizing the functional and cross-linking monomers, methacry

Forest fires are one of the main disturbance agents in boreal and temperate ecosystems. To decipher large-scale temporal and spatial patterns of past fire activity in Scandinavia, we analysed the synchronicity of dendrochoronologically reconstructed fire events in a large network of sites (n=62; 3296 samples, 392 individual fire years) covering a wide geographical gradient (56.5-67.0 degrees N and

The open XML format mzML, used for representation of mass spectrometry (MS) data, is pivotal for the development of platform-independent MS analysis software. Although conversion from vendor formats to mzML must take place on a platform on which the vendor libraries are available (i.e. Windows), once mzML files have been generated, they can be used on any platform. However, the mzML format has tur

Water sorption calorimetry has been used for characterization of 2D hexagonally ordered mesoporous silica SBA-15. Experimental data on water sorption isotherm, the enthalpy, and the entropy of hydration of SBA-15 are presented. The results were compared with previously published results on MCM-41 obtained using the same technique. The water sorption isotherm of SBA-15 consists of four regimes, whi

Increasing the understanding of a proteome and how its protein composition is affected by for example different diseases, such as cancer, has the potential to improve strategies for early diagnosis and therapeutics. The Global Proteome Survey or GPS is a method that combines mass spectrometry and affinity enrichment with the use of antibodies. The technology enables profiling of complex proteomes

Objective To evaluate the specificity of expression patterns of cell-free circulating microRNAs (miRNAs) in systemic lupus erythematosus (SLE). Methods Total RNA was purified from plasma, and 45 different specific, mature miRNAs were determined using quantitative reverse transcriptionpolymerase chain reaction assays. A total of 409 plasma samples were obtained from 364 different patients with SLE,