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Background. The Gross Motor Function Measure (GMFM) is the instrument most commonly used to measure gross motor function in children with cerebral palsy (CP). Different scoring options have been developed, and their measurement properties have been assessed. Limited information is available regarding longitudinal construct validity. Objective. The objective of this research was to study the longit
Persons who stutter often report their stuttering is influenced by emotional reactions, yet the nature of such relation is still unclear. Psychophysiological studies of stuttering have failed to find any major association between stuttering and the activity of the sympathetic nervous system. A review of published studies of heart rate in relation to stressful speech situations indicate that adults
We report on differential conductance measurements on a gold double-dot structure at 4.2 K. The two dots were connected in series by tunnel junctions formed by atomic force microscopy manipulation of nanodisks. The tunnel junctions were made strongly asymmetric. The characteristic honeycomb-shaped charging diagram separating different Coulomb blockade regions of well-defined occupancy of electrons
Let Q be a complex analytic manifold of dimension n with a hermitian metric and C-infinity boundary, and let rectangle = deltadelta* + delta* delta be the self-adjoint delta-Neumann operator on the space L-0,q(2) (Omega) of forms of type (0, q). If the Levi form of deltaOmega has everywhere at least n - q positive or at least q+ I negative eigenvalues, it is well known that Ker rectangle has finit
Reasonably correct phylogenies are fundamental to the testing of evolutionary hypotheses. Here, we present phylogenetic findings based on analyses of 67 complete mammalian mitochondrial (mt) genomes. The analyses, irrespective of whether they were performed at the amino acid (aa) level or on nucleotides (nt) of first and second codon positions, placed Erinaceomorpha (hedgehogs and their kin) as th
The feasibility and scalability of extraction in detergent-based aqueous two-phase systems for the separation of proteins from culture broth is demonstrated. At the same time the large-scale production of a fusion protein and the influence of cultivation scale on the efficiency of separation were investigated. An amphiphilic fusion protein EGIcore-HFBI was chosen, consisting of the catalytic core
Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescenc
Time series analysis of herbivore data with weather included as covariate is commonly used as a mean to shed light on the state and ecology of the studied population. Conclusions about the herbivore population are drawn from statistical parameter values and presence/absence in the most parsimonious model. However, this procedure is only reliable if the statistical parameters have general interpret
We give a simple algorithm that can find biased linear approximations of nonlinear building blocks. The algorithm is useful if the building block is relatively small and exhaustive search is possible. Instead of searching all possible linear relations individually, we show how the most biased relation can be found in just a few steps. As an example we show how we can find a biased relation in the
Objective: To develop calculation models, using Western immunoblot, as a tool for the estimation of proteolytic human aggrecan fragment identity. Method. Seven human aggrecan fragments (calibrators), purified by CsCl gradient centrifugation and identified by Western immunoblot of N- and C-terminals, were used to develop calculation models. The models were used for identification of unknown aggreca
Film formation from latex dispersions with varying concentrations of sodium dodecylsulfate (SDS) and sodium persulfate (NaPS) was studied with a sorption balance. The drying rate decreased significantly at a critical volume fraction of polymer (pc). Under constant drying conditions the pc varied due to differences in particle stabilization. In SDS containing samples, the droplets wetted larger are
We present a fast and accurate method for real-time determination of the absorption coefficient, the scattering coefficient, and the anisotropy factor of thin turbid samples by using simple continuous-wave noncoherent light sources. The three optical properties are extracted from recordings of angularly resolved transmittance in addition to spatially resolved diffuse reflectance and transmittance.
Silicon microchips with immobilized antibodies were used to develop microfluidic enzyme immunoassays using chemiluminescence detection and horseradish peroxidase (HRP) as the enzyme label. Polyclonal anti-atrazine antibodies were coupled to the silicon microchip surface with an overall dimension of 13.1 x 3.2 mm, comprising 42 porous flow channels of 235-mum depth and 25-mum width. Different immob
The division operation is essential in many digital signal processing algorithms. For a hardware implementation, the requirements and constraints on the divider circuit differ significantly with different applications. Therefore, it is not possible to design one divider component having optimal performance and cost for all target applications. Instead, the presented divider has a modular architect
In this article experimental and numerical studies of internal frost resistance of self-compacting and normal concrete, with and without fibres, are outlined. For this purpose self-compacting concrete with low water-cement ratio was studied, with varying amounts of filler, crystalline or sedimentary, different pouring pressures and different mixing procedure with two ages at the start of testing.
• Here, cyanobiont selectivity of epiphytic lichen species was examined in an old growth forest area in Finland. • Samples of the eight lichen species were collected from the same aspen (Populus tremula) and adjacent aspens in the same stand. The cyanobionts of these samples were compared with free and symbiotic Nostoc obtained from other habitats and geographic regions. • Our results, based o
To establish the structure of ferric ions in strongly alkaline (pH > 13) environments, aqueous NaOH solutions supersaturated with respect to Fe(III) and the solid ferric-hydroxo complex salts precipitating from them have been characterized with a variety of experimental techniques. From UV measurements, in solutions of pH > 13, only one kind of Fe(III)-hydroxo complex species was found to be prese
There is a need for efficient methods for estimating trends in spatio-temporal Earth Observation data. A suitable model for such data is a space-varying regression model, where the regression coefficients for the spatial locations are dependent. A second order intrinsic Gaussian Markov Random Field prior is used to specify the spatial covariance structure. Model parameters are estimated using the
