Spatial transcriptomics, a powerful technique that merges sequencing and spatial data, offers unprecedented resolution for studying gene expression within tissues. This study focuses on developing a pipeline to design probes for Multiplexed Error-Robust Fluorescence In Situ Hybridisation (MERFISH) experiments to investigate the spatial distribution of the lung microbiome. Metagenomic data analysis

From Strands to Wires All the genetic information required to develop and maintain an organism is packed tightly into the molecule known as DNA. Determining the order of the four bases, which are the building blocks of DNA, enables the identification of a DNA segment. Modern disease diagnostics use this identification method, known as DNA sequencing, to inspect genetic integrity. Thus, it is vita

Spatial omics allows for the investigation of the Tumor Immune Microenvironment (TIME). Stratifying patients by their TIMEs contributes with insights on tumor immune escape, and help in tumor drug targeting. Tools to investigate such spatial omics approaches suffer from high costs and limited direct clinical applicability. We address this issue by proposing a Machine Learning model which learns to

The aim of this research project was to create a bioinformatic image analysis pipeline for the investigation of lymphocytes in healthy human lungs. The lung is an important organ to study as it provides a fast way for pathogens to enter the body. Therefore, the pulmonary immune system must be further investigated in both healthy and diseased tissue. In order to do comparative studies, we need to c

En fungerande stadsbyggnad och översvämningriskhantering i Quezon City, Filippinerna, ett snabbt växande område med omfattande utbredning av informella bosättningar, är avgörande med tanke på den omfattande risken för översvämningar. Detta examensarbete syftar till att bidra till strategier för stadsplanering i översvämningsdrabbade områden genom att integrera en maskininlärningsmodell med stadsbyUrban development and flood risk management in Quezon City, Philippines, a rapidly urbanising area with extensive informal settlements, are critical given the city's vulnerability to flooding. This thesis aims to contribute to the theoretical framework for urban development in flood-prone areas by integrating a machine-learning model with contextual urban planning. The model uses a Random Fore

This thesis explores reasons of the marginalization of Scanian landscape paintings in the Swedish art history between 1885–1915. The first research question will investigate the construction of the narrative of Swedish art history and how it correlates to the perceived image of Sweden at the turn of the century. The second question examines the consequences this has had for the perception of the S

Peptidläkemedel måste ofta tas som injektioner eftesom peptider är för stora för att kunna absorberas genom celler den orala rutten. Men genom att inkludera absorbtionsförbättrare (surfaktanter) så kan denna absorbtionen för bättras, vilket skulle kunna möjliggöra skapandet av peptidläkemedel i tablettform! Genom att studera interaktionerna mellan peptider och absorbtionsförbättrare så kan man få Therapeutic peptides can often not be formulated as tablets by themselves as they’re prone to enzyme degradation the oral route - they also do not present good enough passage through epithelial cells to be viable for this dosage form. But if formulated together with surfactants, e.g., sodium caprate (C10), the absorption through epithelial cells can be increased. When peptides and absorption enhan

For banks to remain resilient in times of economic instability, and what the Chair of the Supervisory Board of the European Central Bank refers to as; “radical uncertainty”, it is essential for banks to plan for the future. Challenges such as rising interest rates, climate change and geopolitical conflicts require proactive measures. Thus, accurately predicting bank revenue plays a crucial role in

This thesis work was carried out for Höganäs AB, the world leading metal powder manufacturer. The goal was to create a program that automatically compiled logged data from their heat treat ing ovens and present it graphically, based on input in the form of time intervals. The program should also be able to compare outcomes during different time intervals and outcomes in different assignments. Due

I have explored methods of rehearsing pieces in order to play them by heart. I chose to do this study as I wanted to explore if I become more present during my performances if I play by heart. I have read about several methods and tried the ones I found relevant to me which included practice with and without instruments. My choices of methods were visualization, rotating practice schedule and chun

Brasilia, Brazil’s capital, is a city designed under the modernist precepts of the 60s. Its urbanistic plan is defined in four urban scales. Each of these occupies a different location in the city and has its own characteristics, which will be explored in this work. Especially the scale of the city center, which will be the focus of this project. Regional and local analyses were key to understand

The purpose of this study is to give an insight into the archival management of six student nations at Lund University, as well as the general view of the archive amongst the members who are responsible for the student nation archives. The majority of the student nations are well over 300 years old, but there have been few or no studies about the student nations archives, a knowledge gap this stud

With the increasing demands for higher data rates and long-range wireless power transfer, the development of new methods is a necessary step to meet the heightened requirements. One such method is improving the infrastructure for wireless transmission through the use of large intelligent surfaces (LIS). To be one step closer to enabling the construction of such infrastructure, this study examines

The overarching purpose of this thesis was to explore one aspect of Chinese foreign policy known as ‘wolf warrior diplomacy’: a rhetorical and polemic tool wielded by Chinese diplomats to deflect and counter criticism commonly perceived as hostile. This (un)diplomatic approach has bewildered many, which precipitates questions of its use and pertinence especially during a timeline in which China is

This study investigates the influence of the crackdown on democracy in Hong Kong and the Russian invasion of Ukraine on Taiwanese identity building and the approach to cross strait relations among young Taiwanese adults. The study builds on eight in-depth semi-structured interviews with young Taiwanese adults between the age of 25-35. It utilizes constructivist theory of identity building and surv

Metabolism plays a crucial role in an animal’s life as it determines the amount of energy that can be allocated to growth, reproduction, and survival. The strategies an individual adopts to manage its energy budget have direct consequences to its fitness. How an animal allocates its energy will determine whether it can ensure its own survival while having a high reproductive success or neglect one

Food for Thought, or Thought for Food? Obesity affects 12 % of adults worldwide (GBD 2015 Obesity Collaborators, 2017). This number is only expected to increase in the future. Serious risks are associated with obesity such as heart disease, high blood pressure, and type two diabetes (Pantalone et al., 2017). There are not currently effective and safe methods to aid weight loss (Ruban et al., 2019

Hur påverkar anti-TSLP cellkommunikation och inflammation i lungan? Astma är en kronisk andningssjukdom som påverkar miljontals människor runt om i världen. Sjukdomen kännetecknas av luftvägsinflammation, överkänslighet och obstruktion, vilket leder till symtom som väsande andning, andnöd och hosta. Ny forskning visar att läkemedlet Tezepelumab, ett anti-TSLP läkemedel som blockerar TSLP, kan minBackground: Asthma, a prevalent chronic lung disease, affecting millions worldwide, is characterised by airway inflammation and increased smooth muscle mass and constriction, leading to the narrowing of the airway walls. Recent biologics have emerged as promising treatments for asthma. This study focuses on Tezepelumab, a monoclonal antibody targeting TSLP, which has been shown to improve airway h

Merry Go Round: Rolling-circle telomere elongation Imagine a shoelace. At the end of the shoelace, there is a protective piece called an aglet, keeping the fibers together. A human chromosome is a bit like that, too – long and linear, with a characteristic structure at the end called the telomere, which protects the chromosome from instability. With each cell division, a piece of the telomeric

Small cell lung cancer (SCLC) is a highly aggressive form of cancer, characterized by a particularly poor prognosis. Subsequently, it has a very low five-year survival rate of only 6%. At the clinic, patients are either diagnosed at advanced stages where care is more palliative and with few therapeutic options or, when diagnosed at earlier stages, respond very positively to first-line treatments b