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Amplifying bacterial DNA by PCR from human biopsy specimens has sometimes proved to be difficult, mainly due to the low amount of bacterial DNA present. Therefore, nested or semi-nested 16S rDNA PCR amplification has been the method of choice. In this study, we evaluate the potential use of whole genome amplification of total DNA isolated from human colon and rectum biopsy specimens, followed by 1
A new technique based on phosphor thermometry for measurements of two-dimensional gas-phase temperature, was examined as a new laser diagnostic. Calibration of Dy:YAG phosphor was carried out on the surface of a solid. The data were applicable for gas thermometry since the validation of the line intensity ratios method, showed good agreement with both the lifetime method and thermocouple data in a
Sweden, one of the Nordic countries, has a long history of social justice and equality of access to health care. Nursing plays an important role in this and nursing education is of a high standard. The aim of this paper is to describe Sweden's health system and nursing within it, thereby giving Australian nurses information which may generate an interest in, and provide background for, collaborati
Background: Coronary endothelial dysfunction contributes to the pathogenesis of acute coronary syndromes (ACSs). Acute Chlamydia pneumoniae infection has been epidemiologically associated with ACS. In this study, we investigated whether acute C. pneumoniae infection could alter the endothelial vasomotor function of porcine coronary vessels. Methods and results: Twenty pigs, 7–9 kg in weight, were
Basidiomycete fruitbodies growing in coniferous forests along a complex heavy metal pollution gradient in north Sweden were identified and counted over a 4 yr period. The dominant metals in the humus layer were As, Cd, Cu, Pb, and Zn. Pb occurred at the highest levels in a range of 80 to 8500 g g–1 dry wt. Both the number of fruitbodies and the number of fruiting species decreased strongly with in
Cystatin C and the prion protein have been shown to form dimers via three-dimensional domain swapping, and this process has also been hypothesized to be involved in amyloidogenesis. Production of oligomers of other amyloidogenic proteins has been reported to precede fibril formation, suggesting oligomers as intermediates in fibrillogenesis. A variant of cystatin C, with a Leu(68)-> Gln substitutio
Degraded electron beams, as used for intraoperative radiation therapy (IORT) or similar complicated dosimetric situations, have different characteristics compared to conventional electron therapy beams. If international dosimetry protocols are applied in a direct manner to such degraded beams, uncertainties will be introduced in the absorbed dose determination. The Monte Carlo method has been used
This paper presents a first study of the decay constants of the charged, or flavor-off-diagonal, pseudoscalar mesons to two loops for three flavors of sea quarks, in Partially Quenched Chiral Perturbation Theory (PQChPT). Explicit analytical expressions up to order p6 in the momentum expansion are given. The calculations have been performed within the supersymmetric formulation of PQChP
Recent empirical and modelling studies suggest that mid-tropospheric relative humidity (RH) is an important controlling factor of deep atmospheric convection, which appears to be underestimated in present cumulus parametrizations. This indicates the possible presence of shortcomings in the way that entrainment is represented in such parametrizations. This matter was explored in the European Cloud
The purpose of this study was to develop new prediction equations for flow/volume spirometry parameters in asymptomatic, never-smoking adults in Norway, and to assess any differences of these parameters when applying the new and most commonly used equation sets. Flow/volume spirometry was measured according to the American Thoracic Society criteria in 2,792 subjects aged > or = 20 yrs, randomly se
Objective. Recent findings Indicate that platelets not only regulate thrombosis and hemostasis but may also be involved in proinflammatory activities. Herein, we hypothesized that platelets may play a role in sepsis by activating and priming circulating neutrophils for subsequent recruitment Into the lung. Design: Prospective experimental study. Setting. University Hospital Research Unit. Subject.
We investigate how model populations respond to stochastic harvesting in a stochastic environment. In particular, we show that the effects of variable harvesting on the variance in population density and yield depend critically on the autocorrelation of environmental noise and on whether the endogenous dynamics of the population display over- or undercompensation to density. These factors interact
In this paper, we present a novel method for joint estimation of the fundamental frequency and order of a set of harmonically related sinusoids based on the MUltiple SIgnal Classification (MUSIC) estimation criterion. The presented method, termed HMUSIC, is shown to have an efficient implementation using fast Fourier transforms (FFTs). Furthermore, refined estimates can be obtained using a gradien
Three automatic naming tasks (Wiig & Nielsen, 1999) were administered to 60 normal functioning adults. The mean time required for naming 40 single-dimension (colors, forms, numbers, and letters) and 40 dual dimension stimuli (color-form, color-number, and color letter combinations) were compared in young (17-38 yr.) and older (40-68 yr.) men and women. Analysis of variance for the combined gro
Catalytic metal sites in enzymes frequently have second-sphere carboxylate groups that neutralize the charge of the site and share protons with first-sphere ligands. This gives rise to an ambiguity concerning the position of this proton, which has turned out to be hard to settle with experimental, as well as theoretical, methods. We study three such proton-transfer reactions in two proteins and sh
In many gel applications the swelling and shrinking kinetics are very important, e.g. in controlled/slow release, where the kinetics determine the rate of out-diffusion of the active component, and in gel extraction where the gel is swollen and shrunk several times. In this study swelling kinetics of poly(N-isopropylacrylamide) gel (NiPAAm gel) was determined by monitoring the swelling process usi
