Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). In this thesis, we present different metal binding tags, and their af

This paper deals with the joint estimation of spatial mode shapes and temporal frequencies from transient vibrations measured by a uniform circular array of sensors encircling a cross-section of a cylinder. The geometry allows each 2D mode to be interpreted as two real planar waves impinging from mirrored directions with respect to the broadside of a uniform linear array. Algorithms for joint esti

It is shown how tilt anisoplanatism in laser-supported multi-conjugatedadaptive optics (MCAO) systems can be a) predicted from a prioriknowledge, b) compensated using additional natural guide stars (NGS),and c) estimated in real time from control loop data to allow PSFretrieval. As a consequence of the problems associated with retrievingatmospheric tip and tilt from laser guide star (LGS) measurem

As emphasized by Jeremy Bentham and the analytical school, logical consistency is a requirement for rational legislation. For understanding consistency between norms, a logical scrutiny of normative conflicts is needed. In our paper, a framework for the fine structure of such conflicts is introduced and explained. “Normative conflict” is defined relative the framework and different types of confli

Artikeln visar statistik och diskuterar möjligheterna och begränsningarna i äldres tillgänglighet till i IT-samhället under senare delen av 1990-talet. Resultatet från en intervjuundersökning (n=186)visar att äldre IT-användare kan kategoriseras i beroende, akriva och avancerade användare och att detta formas av tillgången, förmågan, förväntningarna, självbilden och dagliga restriktioner (sociala

Popular Abstract in Swedish Magsäcken är ett av kroppens största peptidhormon-producerande organ. Peptid-hormon härifrån är inte bara viktiga för att reglera olika fysiologiska funktioner i magsäcken t.ex. saltsyrasekretion och motilitet, utan också för andra funktioner i kroppen. Fortfarande vet vi mycket lite om de hormon som produceras i magsäcken. Några av de hormon man identifierat har okändaThe novel gastric hormone ghrelin was discovered by virtue of its ability to release growth hormone (GH) via interaction with the so-called GH-secretagogue receptor (GHS-R). Earlier only synthetic agents were known to bind with the GHS-R, hence, ghrelin is the first identified endogenous ligand. GHS-R occurs in the pituitary and hypothalamus but also in several peripheral tissues, suggesting the i

The Java programming language, being a portable and safe object-oriented language, has gained much interest among embedded and real-time systems developers. However, standard Java implementations exhibit problems with performance, memory footprint, and predictability. The question is then, are these limitations inherent in the technology? Reviewing run-time aspects and the possibility to compile

This note is a short discussion on how to present archaeological reconstructions to the public. The author, together with 6 other researchers and authors, comment on means of presenting the past. The author argues that the approach used should be more focussed on sensory and emotional aspects of life in the past.

The present study of spectra of twelve metal-rich cool dwarf stars, carefully selected in order to cover a range of temperatures ( K), is a follow up on Feltzing & Gustafsson (1998) with the aim to understand the apparent over-ionization and anomalous elemental abundances found by them for the K dwarf stars in their sample. Our method of analysis employs synthetic spectra of the full spectrum

Abstract in Undetermined Allt börjar med frågorna: Det eviga - betyder det något idag? Hur ser den akademiska friheten ut? När blir en samling människor en konstellation och vad innebär sådana sammanslutningar? Några av Sveriges främsta författare, forskare och kulturpersoner diskuterar. De talade orden räcker snart inte till. Istället leder dialogen till ett utbyte av texter. Iderika och egensinn