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Recombinant protein development requires not only successful expression and purification, but also the recovery of stable and functionally active proteins. In this study, five recombinant proteins were used as case examples to examine key challenges in early-stage recombinant protein production and functional characterization. These included three oat seed lipase 1 variants, OL522, OL118, and OL18
Modern integrated circuits contain extensive on-chip instrumentation—Ring Oscillators (ROs), temperature sensors, voltage monitors, and delay-measurement circuits—originally intended for reliability monitoring and process calibration. The same physical sensitivity that makes these instruments effective sensors also makes them potential side-channel receivers. An adversary who can place or exploit
This thesis presents the design and schematic-level simulation of a highly linear mixer-first receiver front-end implemented in CMOS, targeting improved robustness against strong input signals. The proposed architecture employs a current-mode passive mixer directly loaded by a low-impedance transimpedance amplifier (TIA) in order to minimize the voltage swing at the mixer output and thereby enhanc
Microvias are key enablers of next-generation high-speed and high-density electronic products. However, their signal-integrity performance can be affected by manufacturing-induced non-idealities, including tapered sidewalls caused by laser drilling and positional staggering caused by fabrication tolerances. This thesis investigates the impact of microvia vertical geometry and manufacturing-induced
As we have become more modern, we have become more reliant on electronic devices, containing micro- and nanosize components. Coming along with more advanced and complex manufacturing methods have become more advanced, they bear plenty of inconveniences, most commonly the impossibility of printing on surfaces with arbitrary surface curvature. This issue is resolved by the reflow transfer printing m
Workplace sexual harassment is believed to cement existing gender-based oppression and imbalanced power structures in which women are subordinate. As a widespread global issue,it has become increasingly urgent in contemporary China. The British television drama Douglas Is Cancelled released in 2024 offers a valuable case for analysing gendered power relations by centring around workplace sexual ha
Sweden has experienced pronounced long-run fluctuations in fertility since the mid-twentieth century, alongside persistent within-year seasonality in births. While many fertility movements can be interpreted ex post in relation to economic conditions, cohort size, and institutional reforms, a central practical challenge is ex ante: when does an emerging decline become statistically detectable as t
This thesis aims to develop and evaluate a fast design and optimisation workflow for corrugated tubes used in tubular heat exchangers by combining computational fluid dynamics (CFD) simulations and machine learning. A steady-state Reynolds-averaged Navier-Stokes (RANS) model was established to simplify the simulation process and reduce computational time. Based on the selected CFD setup, a CFD dat
Many circuits in integrated-circuit design require a time-shifted or multi-phase clock. In time-interleaved ADCs, multiple phases enable a higher effective aggregate sampling rate without increasing the individual clock rate. In contrast, in SerDes links, bit throughput increases while keeping each lane at a lower base clock. In 5G and 6G wireless equipment, multiple phases can be used to drive a
In recent years, the Chinese city of Dali has been intensely romanticised on digital platforms such as Rednote (Xiaohongshu) as a flawless sanctuary of slow living. This digital utopification emerges as a collective response to systemic urban hypercompetition and burnout. Rather than verifying the empirical truth of this destination image, this thesis investigates what utopification actively achie
Syftet med denna studie var att undersöka sambandet mellan upplevd stress, miljöbelastning och olika former av självtillit inom tre specifika domän; professionell självtillit, social självtillit och romantisk självtillit. En kvantitativ ansats användes för att utforska frågeställningarna: “Vilket samband finns det mellan upplevd miljöbelastning, romantisk självtillit, social självtillit och profesThe purpose of this study was to investigate the relationship between perceived stress, environmental strain, and different forms of self-reliance within three specific domains; professional self-reliance, social self-reliance, and romantic self-reliance. A quantitative approach was used to explore the research questions: “What is the relationship between perceived environmental strain, romantic s
Denna studie syftade till att undersöka om det finns ett samband mellan social status, självkänsla och FoMO i relation till upplevd upptagenhet. Den undersökta modellen byggde på hypotesen att låg social status, låg självkänsla och hög FoMO korrelerar med en hög grad av upplevd upptagenhet. Relationerna mellan de oberoende variablerna inom denna specifika modell baserades på tidigare forskning, meThis study aimed to examine if there is an association between social status, self-esteem and FoMO on perceived busyness. The model studied was built around the hypothesis that low social status, low self-esteem and high FoMo correlates with a high perceived busyness. The relationships between the independent variables within this specific model were based on previous research, but the association
The formation of our Galaxy has long been a rewarding area of research within astronomy. A large part of analysis includes using kinematics to separate the different structures in the Milky Way and also to discover accreted stars. While it is highly useful to understand the motions of populations there are systematic errors that follow, due to the difficulties of getting high quality data for them
The system around the star Beta Pictoris is often referred to as the poster child of exoplanet research. However, the planetary system contains many more interesting components inside its large protoplanetary disks. When examining the stellar spectrum, long-lasting absorption lines near the Ca II lines of the star have been observed. While these absorption lines resemble those of transiting exocom
Plate heat exchangers are widely used in industry due to their high thermal efficiency and versatility, compact design and ease of maintenance. In gasketed plate heat exchangers (GPHEs), the reliability of the system strongly depends on the adhesion between the elastomeric gasket and the metallic plate. Although GPHEs can be periodically opened and cleaned to restore heat transfer efficiency, cle
Uppsatsen behandlar vilka problem som uppstår när begreppet ”loss” definieras i aktieöverlåtelseavtal reglerade av svensk rätt vid företagstransaktioner. Inledningsvis undersöks hur ”loss” definieras i praktiken genom ett empiriskt material bestående av definitioner insamlade från delägare på större affärsjuridiska byråer samt intervjuer med delägare verksamma inom företagstransaktioner. UndersöknThis thesis examines the problems that arise when the concept of “loss” is defined in share purchase agreements governed by Swedish law in the context of corporate transactions. Initially, the study examines how “loss” is defined in practice through empirical data consisting of definitions collected from partners at major corporate law firms, as well as interviews with partners active in corporate
Denna uppsats prövar den enskildes rättsliga ställning i en automatiserad förvaltning genom att analysera hur myndighetsövergripande beslutsmodeller förhåller sig till dataskyddsförordningens krav på skydd mot automatiserat beslutsfattande. Analysen genomförs med utgångspunkt i ett rättssäkerhetsperspektiv och har särskilt fokus på hur automatiserad profilering i ett inledande led samverkar med efThis thesis examines the legal position of the individual in an automated public administration by analyzing to what extent fragmented decision-making models can be reconciled with the requirements of the General Data Protection Regulation (GDPR) regarding protection against automated decision-making. The analysis is conducted from a legal certainty perspective and focuses in particular on how aut
Synergies are frequently cited as a primary rationale for mergers and acquisitions, yet they are often overestimated in the pre-acquisition phase and inadequately translated into realised value. While prior research has extensively examined the financial valuation of synergies, less attention has been given to the organisational processes through which synergies are identified, evaluated and commu
This thesis investigates how reliance on ONVIF certification, a global interoperability standard in the network video industry, influences how a large technology company organises its internal organisational structures, roles, responsibilities and resource allocation. Technical standards such as ONVIF, with adherence validated through certification processes, are widely used to promote interoperab
