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Immobilized coenzymes
The Synthesis of Three AMP-Analogues: N6-(6-Aminohexyl)-adenosine 5′-Monophosphate, N6-(6-Aminohexyl)-adenosine 2′,5′-Bisphosphate, and N6-(6-Aminohexyl)-adenosine 3′,5′-Bisphosphate and Their Application as General Ligands in Biospecific Affinity Chromatography
Phosphorylation of 6‐chloropurine riboside with phosphorus oxychloride and phosphorus trichloride gave a mixture of the two isomers, 6‐chloropurine‐riboside 2′,5′‐bisphosphate and 6‐chloropurine‐riboside 3′,5′‐bisphosphate. Reaction with 1,6‐diaminohexane followed by resolution of the isomeric mixture on a Dowex 1‐X2 column yielded N6‐(6‐aminohexyl)‐adenosine 2′,5′‐bisphosphate and N6‐(6‐amin
The preparation and characterisation of a water-soluble coenzymically active dextran-NAD+
A New Immobilized NAD+ Analogue, Its Application in Affinity Chromatography and as a Functioning Coenzyme
Alkylation of NAD+ with iodoacetic acid followed by alkaline rearrangement gave N6-carboxymethyl-NAD+. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD+- N6-[N-(6-aminohexyl)-acetamide]. The coenzymic activity of the two derivatives was tested with malate dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The efficiency relative to unsubstituted NAD+ was i
On Adenine Nucleosides for Affinity Chromatography
Preparation of a NAD(H)-polymer matrix showing coenzymic function of the bound pyridine nucleotide
To a Sepharose gel the pyridine nucleotide NAD(H) has been bound using dicyclohexyl carbodiimide. In order to improve the steric availability of the nucleotide for added soluble enzymes such as dehydrogenases, a spacer molecule, ε-amino caproic acid, was inserted between the carbohydrate matrix and the nucleotide. The obtained preparation contained 56 μmoles NAD+/g dry polymer. The obtained
Cadmium and lead impact on biological phosphorus removal : metal partition and adsorption evaluation in wastewater treatment processes
Understanding the effect and partition of metals in wastewater treatment plants (WWTPs) can aid in designing processes to reduce metal emissions. This study focused on the effects of cadmium and lead, exposed alone (Cd or Pb) and in combination (Cd + Pb), on the enhanced biological phosphorus removal (EBPR) process at lab-scale. Parameters related to the metabolism of propionate, phosphorus (P), p
Advanced Monitoring of H2S Injection through the Coupling of Reactive Transport Models and Geophysical Responses
Hydrogen sulfide (H2S), an environmentally harmful pollutant, is a byproduct of geothermal energy production. To reduce the H2S emissions, H2S-charged water is injected into the basaltic subsurface, where it mineralizes to iron sulfides. Here, we couple geophysical induced polarization (IP) measurements in H2S injection wells and geochemical reactive transport models (RTM) to monitor the H2S stora
Superporous Agarose—A New Material for Chromatography
Superporous particles contain two sets of pores, normal diffusion pores and very large flow pores. If such particles are used chromatographically, part of the flow will pass through each particle. This leads to a rapid equilibration between the stationary and the mobile phases, even if the superporous particle has a large outer diameter. Superporous particles are thus chromatographically efficient
Screening for prostate cancer : evidence, ongoing trials, policies and knowledge gaps
Long-term screening with serum prostate-specific antigen (PSA) and systematic prostate biopsies can reduce prostate cancer mortality but leads to unacceptable overdiagnosis. Over the past decade, diagnostic methods have improved and the indolent nature of low-grade prostate cancer has been established. These advances now enable more selective detection of potentially lethal prostate cancer. This n
Introduction—on tools, bit by bit
This thematic issue of the Journal of Digital History focuses on different digital tools that are used to study, explore, and narrate the past. Our idea has been to collect a broad range of articles that demonstrate and critique new ways of exploring the past through computational means—all in order to open up and problematize the use of digital methods in historical research, including analytical
Impact of extruded mortar joints on the hygrothermal performance of brick veneer walls : A probabilistic study
Mortar extrusion during bricklaying, resulting from suboptimal workmanship, may act as a bridge linking clay brick masonry claddings and adjacent layers (e.g., insulation or weather-resistant barriers), thereby, facilitating water penetration into the adjacent layer owing to wind-driven rain (WDR). Despite the significant effect of water penetration on the hygrothermal performance of building enve
A Literature Review of Probabilistic Hygrothermal Assessment for Building Envelopes
This study presents a state-of-the-art review of the evolution of probabilistic hygrothermal assessments of building envelopes. First, relevant literature was identified by employing a systematic methodology, including scoping and systematic searches, quality assessment, and study analysis. The analysis, based on 143 selected publications, provides statistical insights into uncertainty propagation
No gadolinium K‐edge detected on the first clinical photon counting computed tomography scanner: Response to Dr. Liu and Dr. Noel
Preparation and application of polymer-entrapped enzymes and microorganisms in microbial transformation processes with special reference to steroid 11-β-hydroxylation and Δ1-dehydrogenation
Fungal cells from Curvularia lunata were entrapped in a crosslinked polyacrylamide gel. The gel-cells obtained as granules were applied in the microbial transformation of Reichstein compound S leading to cortisol through an 11-β-hydroxylation step. Some kinetic studies of this conversion using gel-cells were carried out. In addition, it was shown that gel-cell granules which had lost part o
Leder det nya blåkortet till ett mer attraktivt EU?
Vilken framtid har konflikter i den svenska modellen?: Organisationsgrad, kollektivavtal och konfliktfrekvens
Nästan jobb? : Betydelser av kön, plats och tid i den liminala tillvaron som influencer
Influencers befinner sig i epicentrum av digitalisering och motstridiga arbetsideal. En industrikapitalistisk idealisering av (manligt) lönearbete dröjer sig kvar, men utmanas av en nyliberal (postfeministisk) tilltro till individens (inte minst kvinnans) drivkraft att som entreprenör styra livet mot framgång och lycka. Här undersöks hur tolv kvinnliga mikro-influencers förhåller sig till könade nThe feminine-coded work as an influencer has emerged as young women have transformed their personal life stories and self- representations on social media into digital businesses. Despite similarities with other forms of media production, the concept of work is vague, both in the public’s perception of what influencers do and for influencers themselves. It is challenging to differentiate between w