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Here we report that the low-voltage-dependent T-type calcium (Ca2+) channel Cav3.2, encoded by the CACNA1H gene, regulates neuronal differentiation during early embryonic brain development through activating caspase-3. At the onset of neuronal differentiation, neural progenitor cells exhibited spontaneous Ca2+ activity. This activity strongly correlated with the upregulation of CACNA1H mRNA. Cells

Neuroblastoma (NB) is an embryonic tumor of the peripheral nervous system and one of the most common solid cancers in infants. Mutations in the Anaplastic lymphoma tyrosine kinase (ALK) gene are common in NB. To study the contribution of ALK mutations in NB initiation and progression, we reprogrammed fibroblasts from two related NB patients carrying germline mutations in ALK (R1275Q) using non-int

Schizophrenia is a complex disorder with clinical manifestations in early adulthood. However, it may start with disruption of brain development caused by genetic or environmental factors, or both. Early deteriorating effects of genetic/environmental factors on neural development might be key to described disease causing mechanisms. Establishing cellular models with cells from affected individual u

Astrocytes have important functions in the brain and their deregulation may cause disease. Current ways to derive astrocytes from pluripotent stem cells are labor, time, and resource intensive, but in this issue of Stem Cell Reports, Li et al. present a faster method to produce functional astrocytes using transcription factors.

Cell-based models of the blood-brain barrier (BBB) are important for increasing the knowledge of BBB formation, degradation and brain exposure of drug substances. Human models are preferred over animal models because of interspecies differences in BBB structure and function. However, access to human primary BBB tissue is limited and has shown degeneration of BBB functions in vitro. Human induced p

In vitro modeling of complex diseases is now a possibility with the use of patient-derived induced pluripotent stem (iPS) cells. Their stem cell properties, including self-renewal and their potential to virtually differentiate into any cell type, emphasize their importance as a translational tool for modeling disorders that so far have been limited by the unavailability of primary cell lines, anim

In massive multiple-input multiple-output (MIMO) systems, the large size of channel state information (CSI) matrix significantly increases the computational complexity of uplink detection and size of required memory to store the channel data. To address these challenges, we propose to perform detection in the angular domain, where the channel information can be presented in a more condensed way. T

Introduction: In contrast to the intake of sugar-sweetened beverages (SSBs), the evidence linking added sugar intake to the risk of cardiometabolic disease (primarily referring to cardiovascular disease and type 2 diabetes (T2D)) is contradictory. Aim: The aim of this thesis is to elucidate the role of added sugar intake in the risk for cardiometabolic diseases. To obtain further understanding of

In vivo studies of human brain cellular function face challenging ethical and practical difficulties. Animal models are typically used but display distinct cellular differences. One specific example is astrocytes, recently recognized for contribution to neurological diseases and a link to the genetic risk factor apolipoprotein E (APOE). Current astrocytic in vitro models are questioned for lack of

The BRCA1 protein, one of the major players responsible for DNA damage response has recently been linked to Alzheimer's disease (AD). Using primary fibroblasts and neurons reprogrammed from induced pluripotent stem cells (iPSC) derived from familial AD (FAD) patients, we studied the role of the BRCA1 protein underlying molecular neurodegeneration. By whole-transcriptome approach, we have found wid

Caffeine is a widely consumed psychoactive substance, but little is known about the effects of caffeine stimulation on global gene expression changes in neurons. Here, we conducted gene expression profiling of human neuroepithelial stem cell-derived neurons, stimulated with normal consumption levels of caffeine (3 μM and 10 μM), over a period of 9 h. We found dosage-dependent activation of immedia

Xeno-free and fully defined conditions are key parameters for robust and reproducible generation of homogenous human induced pluripotent stem (hiPS) cells. Maintenance of hiPS cells on feeder cells or undefined matrices are susceptible to batch variances, pathogenic contamination and risk of immunogenicity. Utilizing the defined recombinant human laminin 521 (LN-521) matrix in combination with xen

AIMS/HYPOTHESIS: Accurate prediction of disease progression in individuals with pre-symptomatic type 1 diabetes has potential to prevent ketoacidosis and accelerate development of disease-modifying therapies. Current tools for predicting risk require multiple blood samples taken during an OGTT. Our aim was to develop and validate a simpler tool based on a single blood draw.METHODS: Models to predi

A polymer electrolyte fuel cell (PEFC) is an electrochemical device that converts chemical energy into electrical energy and heat. The energy conversion is simple; however, the multiphysics phenomena involved in the energy conversion process must be analyzed in detail. The gas diffusion layer (GDL) provides a diffusion media for reactant gases and gives mechanical support to the fuel cell. It is a

The study aims to explore the evil eye belief practices among Saraiki mothers in a village in South Punjab, Pakistan. This study unfolds three aspects of the evil eye phenomenon; first, the ‘diagnosis’ of the evil eye that constitute the ineffectiveness of the available modern medicine, second the socio-cultural nexus of folk medical system and unmanageable or unexplained threats, and third the pr

Homelessness is a growing global problem. Homelessness produces and reinforces social exclusion. The main reason for homelessness is that there is a shortage of housing available to people with low incomes. Counteracting homelessness requires housing policy initiatives that make it possible for people with low incomes to have access to housing. At the same time people who are homeless must receive

Background: Sepsis, the life-threatening organ dysfunction due to dysregulated host response to an infection, is a medical emergency. Early diagnosis and treatment are important factors to prevent mortality and morbidity. However, sepsis can be diffuse and difficult to interpret. Therefore, it is important to define models for early identification applicable in the Emergency Department (ED), in or

Performing fundamental operando catalysis studies under realistic conditions is a key to further develop and increase the efficiency of industrial catalysts. Operando X-ray photoelectron spectroscopy (XPS) experiments have been limited to pressures, and the relevance for industrial applications has been questioned. Herein, we report on the CO oxidation experiment on Pd(100) performed at a total pr

Soil cation exchange capacity (CEC) strongly influences the chemical, physical, and biological properties of soil. As the direct measurement of the CEC is difficult, costly, and time-consuming, the indirect estimation of CEC from chemical and physical parameters has been considered as an alternative method by researchers. Accordingly, in this study, a new hybrid model using a support vector machin