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Development of an automated flow-based spectrophotometric immunoassay for continuous detection of zearalenone

Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an automated sequential injection system. Time for an assay cycle was 18 Min. Under op

Mothers' experiences of pain during breastfeeding in the early postnatal period : A short report in a Swedish context

Objectives: The objective was to investigate the prevalence of mothers who experienced pain during breastfeeding in the early postnatal period and to describe associated factors. Methods: Medical records of 987 mothers and their babies were scrutinized. Chi-square Test, McNemar's test, and relative risk with a 95% confidence interval were applied for analysis. Results: During the in-hospital postn

HERFD-XANES probes of electronic structures of ironII/IIIcarbene complexes

Iron centeredN-heterocyclic carbene (Fe-NHC) complexes have shown long-lived excited states with charge transfer character useful for light harvesting applications. Understanding the nature of the metal-ligand bond is of fundamental importance to rationally tailor the properties of transition metal complexes. The high-energy-resolution fluorescence detected X-ray absorption near edge structure (HE

Proteomic changes after fertilization and before first cleavage in rainbow trout Oncorhynchus mykiss

In most vertebrates, the development of a new individual starts with fertilization of the oocyte by the spermatozoon. A label-free quantification strategy was applied to investigate proteomic changes after fertilization and before first cleavage in the egg of rainbow trout. Samples were obtained from oocyte and fertilized eggs at 50, 200 min, and 15 h after fertilization at 4 °C. Significant chang

Energy 4 all? Investigating gendered energy justice implications of community-based micro-hydropower cooperatives in Ethiopia

More than 70% of the population in Ethiopia lack access to electricity and thus rely on conventional sources of energy such as biomass that is associated with negative consequences on health and the environment. Decentralized community-based micro-hydropower plants (MHPs) are being utilized as effective means to transition to modern low-carbon energy systems providing access to electricity to comm

Climate change, water quality and water-related challenges : A review with focus on Pakistan

Climate variability is heavily impacting human health all around the globe, in particular, on residents of developing countries. Impacts on surface water and groundwater resources and water-related illnesses are increasing, especially under changing climate scenarios such as diversity in rainfall patterns, increasing temperature, flash floods, severe droughts, heatwaves and heavy precipitation. Em

Peptide-Oleate Complexes Create Novel Membrane-Bound Compartments

A challenging question in evolutionary theory is the origin of cell division and plausible molecular mechanisms involved. Here, we made the surprising observation that complexes formed by short alpha-helical peptides and oleic acid can create multiple membrane-enclosed spaces from a single lipid vesicle. The findings suggest that such complexes may contain the molecular information necessary to in

An unbiased approach to compressed sensing

In compressed sensing a sparse vector is approximately retrieved from an underdetermined equation system Ax = b. Exact retrievalwouldmean solving a large combinatorial problem which is well known to be NP-hard. For b of the form Ax0 + ϵ, where x0 is the ground truth and ϵ is noise, the 'oracle solution' is the one you get if you a priori know the support of x0, and is the best solution one could h

Immune phenotypes of nasopharyngeal cancer

Nasopharyngeal cancer (NPC) features intralesional immune cells, but data are lacking on presence/distribution of T-cells and dendritic cells (DCs). Based on intralesional distribution of lymphocytes, a series of NPC biopsies (n = 48) were classified into “inflamed”, “excluded”, and “deserted” phenotypes. In addition, CD8+ T-cells and CD207+ DCs were quantified. The data were analyzed in relation

Modelling maize yield and water requirements under different climate change scenarios

African countries such as Nigeria are anticipated to be more susceptible to the impacts of climate change due to large dependence on rainfed agriculture and to several uncertainties in the responses of crop production to climate change. The impacts of climate change on crop water requirements (CWR), irrigation water requirements (IWR), yields and crop water productivity (CWP) of rainfed maize in O

Validity and reliability of the Turkish Occupational Balance Questionnaire (OBQ11-T)

Background: Occupational balance (OB) is an important concept in occupational therapy and is considered as an essential component of health and well-being. Aim: The aim of this study was to investigate the validity and reliability of the Turkish version of the Occupational Balance Questionnaire-11 (OBQ11-T). Materials and methods: The OBQ11-T was administered to 116 individuals for construct valid

Thoughts on the interpretation of positive photopatch test reactions

BACKGROUND: Positive photopatch test reactions are classified according to the International Contact Dermatitis Group. The various reaction patterns are interpreted to represent patterns such as contact allergy, photocontact allergy, photoaugmentation, and photoinhibition. OBJECTIVE: To investigate whether there are any weaknesses in the interpretation of reaction patterns. MATERIALS & METHODS

Mucosa-associated invariant T cells link intestinal immunity with antibacterial immune defects in alcoholic liver disease

Background/aims: Intestinal permeability with systemic distribution of bacterial products are central in the immunopathogenesis of alcoholic liver disease (ALD), yet links with intestinal immunity remain elusive. Mucosa-associated invariant T cells (MAIT) are found in liver, blood and intestinal mucosa and are a key component of antibacterial host defences. Their role in ALD is unknown. Methods/de

On long-term outcome of anastomotic leakage after anterior resection for rectal cancer

Background: Anterior resection has the benefit of enabling bowel continuity, but suffer from a common complication in anastomotic leakage.There is a paucity in previous research of long-term implications of anastomotic leakage after anterior resection.Aims: To gain general knowledge of the morbidity related to anastomotic leakage after anterior resection for rectal cancer, specific aims were:I. ToBackground: Anterior resection has the benefit of enabling bowel continuity, but suffer from a common complication in anastomotic leakage.There is a paucity in previous research of long-term implications of anastomotic leakage after anterior resection.Aims: To gain general knowledge of the morbidity related to anastomotic leakage after anterior resection for rectal cancer, specific aims were:I.To

Restoration of the electrocardiogram during mechanical cardiopulmonary resuscitation

Objective: An artefact-free electrocardiogram (ECG) is essential during cardiac arrest to decide therapy such as defibrillation. Mechanical cardiopulmonary resuscitation (CPR) devices cause movement artefacts that alter the ECG. This study analyzes the effectiveness of mechanical CPR artefact suppression filters to restore clinically relevant ECG information. Approach: In total, 495 10 s ECGs were

Supply chain uncertainties of small-scale coffee husk-biochar production for activated carbon in Vietnam

Between 2014 and 2019, an innovative small-pyrolysis system (the PPV300) using agrowaste as feedstock was transferred and adapted from Switzerland to Vietnam by a United Nations Industrial Development Organization (UNIDO) project. The coffee husk resulting from the processing of coffee beans is usually disposed of in Vietnam or burned inefficiently to dry coffee beans. Small-scale pyrolysis of cof

The Gaia -ESO Survey: Calibrating the lithium-age relation with open clusters and associations: I. Cluster age range and initial membership selections

Context. Previous studies of open clusters have shown that lithium depletion is not only strongly age dependent but also shows a complex pattern with other parameters that is not yet understood. For pre- and main-sequence late-type stars, these parameters include metallicity, mixing mechanisms, convection structure, rotation, and magnetic activity. Aims. We perform a thorough membership analysis f

A new equation of state applied to planetary impacts : II. Lunar-forming impact simulations with a primordial magma ocean

Observed FeO/MgO ratios in the Moon and Earth are inconsistent with simulations done with a single homogeneous silicate layer. In this paper we use a newly developed equation of state to perform smoothed particle hydrodynamics simulations on the lunar-forming impact, testing the effect of a primordial magma ocean on Earth. This is investigated using the impact parameters of both the canonical case

Rocklines as cradles for refractory solids in the protosolar nebula

In our solar system, terrestrial planets and meteoritical matter exhibit various bulk compositions. To understand this variety of compositions, formation mechanisms of meteorites are usually investigated via a thermodynamic approach that neglects the processes of transport throughout the protosolar nebula. Here, we investigate the role played by rocklines (condensation/sublimation lines of refract