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Flow immunochemical bio-recognition detection for the determination of interleukin-10 in cell samples

On- and off-line heterogeneous non-competitive flow immunoassays for the determination of Interleukin-10 are described. The sample containing IL-10 is mixed, either on-line in a reaction coil or off-line in a test tube, with fluorescent labelled anti-IL-10 antibodies to form an antibody-antigen complex. The labelled unbound antibodies are trapped on an immobilized IL-10 column whereas the IL-10-an

Implementation of a protein profiling platform developed as an academic-pharmaceutical industry collaborative effort

As much attention has devoted to the proteome research during the last few years, biomarker discovery has become an increasingly hot area, potentially enabling the development of new assays for diagnosis and prognosis of severe diseases. This is the field of research interest where efforts originating from both academic and industrial groups should jointly work on solutions. In this paper, we woul

On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes

An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perfusion liquid used for sampling hydrophobic eicosanoids in biological systems was also studied. The total time for one an

Development of an offline noncompetitive flow immunoassay for the determination of interleukin-8 in cell samples

A noncompetitive flow immunoassay system (FIA) for the analysis of interleukin-8 (IL-8) in cell samples was developed. Affinity interaction assays based on offline incubation of excess labeled antibodies and antigen (IL-8) were carried out. The residual unbound labeled antibody was trapped in an immunoaffinity column with immobilized IL-8 while the immunocomplex, labeled antibody/IL-8, was detecte

General and specific contextual effects in multilevel regression analyses and their paradoxical relationship : A conceptual tutorial

To be relevant for public health, a context (e.g., neighborhood, school, hospital) should influence or affect the health status of the individuals included in it. The greater the influence of the shared context, the higher the correlation of subject outcomes within that context is likely to be. This intra-context or intra-class correlation is of substantive interest and has been used to quantify t

Distinctive peri-luminal presence of agrin in murine and human carotid atherosclerotic plaques

The clinical consequences of arterial atherosclerotic lesions depend, apart from their size, on their composition of cellular and extracellular components. While an intact endothelium at the interface of atherosclerotic plaques towards the blood can prevent its erosion, underlying smooth muscle cells within the plaque can reduce the risk of plaque ruptures, due to the deposition of stabilizing ext

An experimental and theoretical study of a heptacoordinated tungsten(VI) complex of a noninnocent phenylenediamine bis(phenolate) ligand

[W(N2O2)(HN2O2)] (H4N2O2 = N,N′-bis(3,5-di-tert-butyl-2-hydroxyphenyl)-1,2-phenylenediamine) with a noninnocent ligand was formed by reaction of the alkoxide precursor [W(eg)3] (eg = the 1,2-ethanediolate dianion) with two equivalents of ligand. The phenol groups on one of the ligands are completely deprotonated and the ligand coordinates in a tetradentate fashion, whereas the other ligand is trid

The pro-inflammatory effects of particulate matter on epithelial cells are associated with elemental composition

Background: Adverse health effects of particulate matter (PM) vary with chemical composition; however, evidence regarding which elements are the most detrimental is limited. The roof space area provides an open and stable environment for outdoor PM to settle and deposit. Therefore, this study used roof space PM samples as a proxy of residential cumulative exposure to outdoor air pollution to inves

Effect of blockage configuration on film cooling with and without mist injection

Film cooling, as an effective protection technology, is widely applied in gas turbines to prevent components like vane blades from the hot mainstream. However, ash and other contaminants contained in the gas easily deposit on blade surfaces, which results in blockages inside the film holes and a reduction of the coverage area of the coolant air. Two blockage configurations (hemispherical and tetra

Medium-term heat load prediction for an existing residential building based on a wireless on-off control system

For district heating systems, prediction of the heat load is a very important topic for energy storage and optimized operation. For large and complex heating systems, most prediction models in previous publications only considered the influence of outdoor temperature, whereas the indoor temperature and thermal inertia of buildings were not included. For an energy-efficient residential building in

New copper(II) salicylaldimine derivatives for mild oxidation of cyclohexane

Abstract: Two new salicylaldiminato-copper(II) complexes, [Cu(L1)2] (1) and [Cu(L2)2] (2) (where HL1= 4 -tert-Butyl-2-[(thiophen-2-ylmethylimino)-methyl]-phenol and HL2= 2 , 4 -Di-tert-butyl-6-[(thiophen-2-ylmethylimino)-methyl]-phenol), endowed with a pendant thiophenyl moiety, were synthesized and characterized using standard spectroscopic techniques (FT-IR, UV-Vis, MS) and elemental analysis. C

Seeking No War, Achieving No Peace: The Conflict over the Siachen Glacier

This paper models “no war, no peace” situations in a game theoretical framework where two countries are engaged in a standoff over a military sector. The first main objective is to identify rational grounds for such situations and, more precisely, explicit equilibria that leads to such situations. It is demonstrated that both countries get the same payoff from being in this continuous state of per

Participation and Losses in Multi-Level Marketing: Evidence from an FTC Settlement

More than 20 million Americans are affiliated with Multi-Level Marketing firms (MLMs), but there is little empirical evidence on who participates in this controversial part of today's labor market. We link data on 350,000 individuals cited in an FTC settlement with one of the largest MLMs to detailed county-level information. We find that participation is greater in areas with higher median income

Integrated Cambrian biostratigraphy and carbon isotope chemostratigraphy of the Grönhögen-2015 drill core, Öland, Sweden

The Grönhögen-2015 core drilling on southern Öland, Sweden, penetrated 50.15 m of Cambrian Series 3, Furongian and Lower–Middle Ordovician strata. The Cambrian succession includes the Äleklinta Member (upper Stage 5) of the Borgholm Formation and the Alum Shale Formation (Guzhangian–Tremadocian). Agnostoids and trilobites allowed subdivision of the succession into eight biozones, in ascending orde

Grassroots practices of citizenship and politicization in the urban : the case of right to the city initiatives in Barcelona

This article aims to produce an analysis of the politicization of the citizens after Spain’s Indignados movement from a citizenship framework. The article suggests that claiming the right to the city involves more than issues of access to urban amenities: it is also about claiming the right to participate in the formation and transformation of the city and the right to appropriate the city center.

Increasing preoperative body size in breast cancer patients between 2002 and 2016 : implications for prognosis

Overweight and obesity are increasing worldwide, but the extent in breast cancer patients is unknown. The two aims were to study secular trends in preoperative body mass index (BMI), waist circumference, and breast volume and their impacts on clinical outcome. BMI, waist circumference, and breast volume were measured preoperatively in 24–99-year-old primary breast cancer patients (n = 640) in Swed

Tracing Multimetal Craftsmanship through Metallurgical debris : Open air workshops and multimetality in Late Iron Age Scandinavia

Metallurgical debris is by far the most informative source material for studying the metal craftsmanship of the past. In comparison to the finished objects, which has attracted far more attention in archaeological research, debris material are more or less confined to the original workshop sites and hence provide direct evidence as to production volume and quality, site organization, artisanal ski