pH dependency of ligand binding to cellobiohydrolase 1 (Cel7A) - Affinity, selectivity and inhibition for designed propranolol analogues
The affinity and enantioselectivity have been determined for designed propranolol derivatives as ligands for Cel7A by capillary electrophoresis (CE) at pH 7.0. These results have been compared to measurements at pH 5.0. In agreement with previous studies, the affinity increased at the higher pH. However, the affinity was not as dependent of the ligand structure at pH 7.0 as at pH 5.0, and the sele