Expression and Purification of BoGal36A Wild-type and W297F Mutant
This study investigated the enzyme BoGal36A, produced by the gut bacterium Bacteroides ovatus. BoGal36A is an alpha-galactosidase, an enzyme that breaks down complex sugars. Unlike most alpha-galactosidases from the GH36 family, BoGal36A appears to cleave internal glycosidic bonds. In this study, the wild-type BoGal36A and mutant W297F was expressed in E. coli BL21 (DE3) cells and purified subsequ
