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Phosphoric acid as a matrix additive for MALDI MS analysis of phosphopeptides and phosphoproteins

Phosphopeptides are often detected with low efficiency by MALDI MS analysis of peptide mixtures. In an effort to improve the phosphopeptide ion response in MALDI MS, we investigated the effects of adding low concentrations of organic and inorganic acids during peptide sample preparation in 2,5-dihydroxybenzoic acid (2,5-DHB) matrix. Phosphoric acid in combination with 2,5-DHB matrix significantly

The Battle Over Belarus : The Rise and Fall of the Belarusian National Movement, 1906-1931

This study examines the rise and fall of the modern Belarusian national movement during the quarter century between 1906, the year when the first Belarusian paper appeared, until its demise around 1931, as a result of political repression in the Soviet Union and Poland. It surveys the emergence of the modern concept of a Belarusian nation, from the first steps towards national consolidation in the

Experimental and numerical investigation of a prototype low NOx gas turbine burner

Clean gas turbine combustion research has gained popularity in power generation and propulsion industry in recent days. Present days, advanced efficient emission (NOx, CO and UHC) reduction combustion technologies are acknowledged for using the lean premixed combustion system. To support continuous development of eco-friendly combustion system, a 4th generation dry low NOx prototype downscaled bur

Attitudes towards hepatitis C and treatment willingness in injection drug users: a follow-up interview study.

Many injection drug users are infected with hepatitis C virus (HCV), yet few are treated. With opiate maintenance treatment, expected life length increases, and the incitement increases to treat HCV in order to avoid long term complications. The aim of the present study was to investigate attitudes towards HCV and HCV treatment willingness among opiate-dependent injection drug users at Malmö needl

Financialisation of Built Environments: Urban Governance, Social Geographies, and Sustainability

This paper is about the ways in which financialisation of built environments hasimpacted upon the politics, social constitution and sustainability of cities. We focus primarily on financialisation of housing, and do so from the methodologicalperspective of variegated financialisation: as a generic process that contextually coevolves with related processes such as neoliberalisation and polarisation

Femtosecond X-Ray Scattering Study of Ultrafast Photoinduced Structural Dynamics in Solvated[Co(terpy)2]2$

We study the structural dynamics of photoexcited [Co(terpy)2]2+ in an aqueous solution with ultrafast x-ray diffuse scattering experiments conducted at the Linac Coherent Light Source. Through direct comparisons with density functional theory calculations, our analysis shows that the photoexcitation event leads to elongation of the Co-N bonds, followed by coherent Co-N bond length oscillations ari

Sertoli cells decrease microglial response and increase engraftment of human hNT neurons in the hemiparkinsonian rat striatum

Sertoli cells (SCs) provide immune protection and nutritive support to the developing germ cells in the testis. Sertoli cells have also been shown to provide immune protection to islets transplanted outside the testes. In this study, the ability of these cells to diminish the infiltration/activation of microglia into a neural graft implanted in the lesioned striatum of a hemiparkinsonian rat was i

Viability and survival of hNT neurons determine degree of functional recovery in grafted ischemic rats

We recently reported behavioral improvements following intrastriatal transplantation of cryopreserved cultured human neuroteratocarcinoma-derived cells (hNT neurons) in rats with cerebral ischemia induced by occlusion of the middle cerebral artery. In the present study, the viability and survival of hNT neurons were evaluated immediately prior to the transplantation surgery and at 3 months post-tr

Preparation of cell suspensions for co-transplantation : methodological considerations

The purpose of the current study was to determine the optimal strategy for preparing cell suspensions for co-transplantation. In the first experiment, the number of Sertoli cell (SC) aggregates and the number of tyrosine hydroxylase positive neurons were compared over time when cell suspensions of Sertoli or ventral mesencephalic cells were kept as a co-suspension mixed at 0 h. Cells from each sus

Trophic effect of porcine Sertoli cells on rat and human ventral mesencephalic cells and hNT neurons in vitro

The poor survival of embryonic dopaminergic (DA) neurons transplanted into patients with Parkinson's disease (PD) has encouraged researchers to search for new methods to affect the short- as well as long-term survival of these neurons after transplantation. In several previous rodent studies Sertoli cells increased survival of islet cells and chromaffin cells when cotransplanted in vivo. The aims