Automated Tracing of Adsorbed DNA Molecules with Curvature Using B-Splines
Barcoding is a coarse-grained optical DNA sequencing method, where DNA molecules are stained with fluorescent dyes to create sequence-dependent patterns similar to barcodes. For DNA adsorbed onto a glass surface, we need to be able to trace out these molecules for barcode extraction. We show that B-splines can be used to approximate the shape of adsorbed DNA molecules with curvature and present a