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Preparation of a minimum PSII core complex from spinach is described, containing four Mn per reaction center (RC) and exhibiting high O2 evolving activity [~4000 mol of O2 (mg of chl)-1 h-1]. The complex consists of the CP47 and CP43 chlorophyll binding proteins, the RC D1/D2 pair, the cytochrome b559 subunits, and the Mn-stabilizing psbO (33 kDa) protein, all present in the same stoichiometric am
Rotational coherent anti-Stokes Raman spectroscopy (CARS) is a well-established spectroscopic technique for thermometry at pre-combustion temperatures an atmospheric pressure. However, at pressures of several MPa, a previous investigation revealed large discrepancies between experimental data and the theoretical model. A re-evaluation has been made of these data (at room temperature and in the ran
A general method for automatically reducing detailed kinetic mechanisms for complex fuels is applied. The method is based on the simultaneous use of sensitivity, reaction-flow, and extended lifetime analyses. The sensitivity analysis detects species to which the overall combustion process is sensitive. Small inaccuracies in calculating these species result in large errors in the characteristic beh
Spontaneous or steroid-induced eosinophil apoptosis occurring in vitro has not been demonstrated in lung tissues in vivo. This study examines cell apoptosis in rat lungs using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) technique and transmission electron microscopy (TEM). After establishing sustained lung edema and eosinophilia by
The formerly monotypic genus Ahtiana (type species: A. sphaerosporella (Müll. Arg.) Goward is shown to include two additional species, A. aurescens (Tuck.) Thell & Randl. and A. pallidula (Riddle) Goward & Thell. All three species are endemic to North America, and are characterized by their greenish yellow upper surface, globose ascospores, and caperatic acid in the medulla. The habitat ec
BACKGROUND: Individuals with chronic depression respond poorly to both medication and psychotherapy. The reasons for the poorer response, however, remain unclear. One potential factor is the presence of comorbid Axis II personality disorders (PDs), which occur at high rates among these patients. METHODS: This study examines the moderating influence of co-occurring PDs, primarily in cluster C, amon
The molecular motion in water of the poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer with the nominal composition EO97PO68EO97 (F127) was investigated with the aid of pulsed field gradient nuclear magnetic resonance (PFG NMR). The signal decays in the PFG experiments have been recorded for 1 wt % F127 in the temperature range from 288 to 313 K and in the concentr
An advanced detection system based on laser-induced fluorescence imaging for capillary electrophoresis (CE) is presented. An optical fiber array was constructed for collection and transportation of the emitted fluorescent light to the charge-coupled device (CCD) camera. The fiber array makes the setup compact compared with a setup where the capillary is imaged through a camera objective. The imagi
We have studied the optical and structural properties of single, self-assembled InP quantum dots (QDs) overgrown with nominally 5 nm of GaInP, using an ultrahigh-vacuum scanning tunneling microscope (STM) operating at low temperatures. The STM is combined with an optical detection system, which allows us to detect the emission from individual quantum dots with high spatial resolution. We find that
We investigate a class of discrete distributions generated by expanding a parametric function in a Lagrange series. There is a close relationship to the class of generalized Poisson distributions, and special cases of our class of distributions arise in the context of random mapping theory. Unbiased estimation is discussed and the results are applied to inference problems in connection with two ra
A REE-bearing fluorosilicate from the Tommot REE-Nb deposit in Yakutia, Russia, described without a name in 1966, is characterized here as a new species, proshchenkoite-(Y), of the vicanite group of borosilicates. Wavelength-dispersive electron probe analyses gave the following empirical formula: (Y3.70REE7.54Ca1.55Na1.16Mn0.77Th0.10Pb0.01)(Sigma 14.83)(Fe0.832+M0.15Ti0.02)(Sigma 1.00)Ca-1.00 (P0.
We present a general model study of surface-enhanced resonant Raman scattering and fluorescence focusing on the interplay between electromagnetic effects and the molecular dynamics. Our model molecule is placed close to two Ag nanoparticles and has two electronic levels. A Franck-Condon mechanism provides electron-vibration coupling. Using realistic parameter values for the molecule we find that a
The aim was to determine whether outcome of unrelated donor transplantation for severe aplastic anemia has improved in recent years and whether this is due to patient selection or better transplant technology. We analyzed 498 patients transplanted during 1990-2005. By running univariate regression models dichotomizing year of transplantation we defined 1998 as the year of the most significant chan
Aim: To conduct a multi-language translation and cross-cultural adaptation of the Intermittent and Constant OsteoArthritis Pain (ICOAP) questionnaire for hip and knee osteoarthritis (OA). Methods: The questionnaires were translated and cross-culturally adapted in parallel, using a common protocol, into the following languages: Czech, Dutch, French (France), German, Italian, Norwegian, Spanish (Cas
In a recent paper,(1) we investigated how the interaction between two adsorbing surfaces in a polymer solution responds to changes of the intrinsic stiffness of the polymer chains. We demonstrated that as the chain rigidity is increased, starting from the fully flexible limit, a free energy barrier develops. In this work, we shall continue our studies of semiflexible polymers but focus on the othe
The sociology of research and knowledge use, argue the authors, could be a way of linking important parts of sociology, such as organization studies, the sociology of science to each other. In the article, they discuss the idea that organizational responses to environments are related to research utilization. Based upon an empirical investigation of city welfare departments, four empirical “utiliz
We investigated the relationship between nitric oxide (NO) and Na+,K+-ATPase (NKA) in the gill of anadromous Atlantic salmon. Cells containing NO-producing enzymes were revealed by means of nitric oxide synthase (NOS) immunocytochemistry and nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) histochernistry, which can be used as an indicator of NOS activity, i.e. NO production. Antibo
