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Affymetrix GeneChip technology and quantitative real-time PCR (Q-PCR) were used to examine changes in gene expression in the adult murine substantia nigra pars compacta (SNc) following lentiviral glial cell line-derived neurotrophic factor (GDNF) delivery in adult striatum. We identified several genes that were upregulated after GDNF treatment. Among these, the gene encoding the transmembrane prot

The number of regulatory RNAs with identified non-canonical structures is increasing, and structural transitions often play a role in their biological function. This stimulates interest in internal motions of RNA, which can underlie structural transitions. Heteronuclear NMR relaxation measurements, which are commonly used to study internal motion, only report on local motions of few sites within t

The presence of ribonucleic acid hairpin structures is important for proper processing of the genetic information in living cells. Recent studies indicate that interactions between platinum based anticancer active metal complexes and hairpin motifs result in a change of their three-dimensional structure combined with a decrease of the melting temperature. We here report a study of the reaction bet

Helicobacter pylori attaches via lectins, carbohydrate binding proteins, to the carbohydrate residues of gastric mucins. Guinea-pigs are a suitable model for a H. pylori infection and thus the carbohydrate composition of normal and H. pylori infected gastric mucosa was investigated by lectin histochemistry. The stomach of all infected animals showed signs of an active chronic gastritis in their mu

Objective - LDL oxidation is believed to play an important role in the development of atherosclerosis, and oxidized LDL particles have been shown to become targets for the immune system. Immunization of animals with oxidized LDL results in reduction of atherosclerosis, suggesting an atheroprotective effect of this immune response. Methods and Results - Using a polypeptide library covering the comp

High spin states in ₆₆Dy isotopes with two protons outside the Z=64 closed shell are discussed. Bands terminating at higher and higher spin values are formed from successive proton excitations across this proton shell. These bands show specific features depending on the number of excited particles. Cranked Nilsson-Strutinsky (CNS) calculations show good agreement with experiment. Specia

Aldose reductase (AR) has been implicated in the pathogenesis of diabetic complications, although the clinical efficacy of AR inhibitors has not been clearly proven. To clarify the pathophysiological role of AR in the heart, we investigated effects of AR inhibitors applied either during the pre-ischemic phase, or during the post-ischemic reperfusion phase on ischemia-reperfusion injury in isolated

Pharmacogenomics is the application of genomic technologies to drug discovery and development, as well as for the elucidation of the mechanisms of drug action on cells and organisms. DNA microarrays measure genome-wide gene expression patterns and are an important tool for pharmacogenomic applications, such as the identification of molecular targets for drugs, toxicological studies and molecular d

We previously described a simplified quantitative hemolytic assay for classical pathway (CP) hemolytic function in serum that has been shown to correlate with the 50% hemolytic complement (CH50) assay. In the present study, we used this assay to compare CP functions; plasma levels of C3, C4, and C3dg; and ratios of C3dg to C3 in healthy individuals and patients with systemic lupus erythematosus (S

A Holocene palaeomagnetic secular variation master curve (FENNOSTACK) and a relative palaeointensity curve (FENNORPIS) for Fennoscandia are presented. These curves were produced by stacking palaeomagnetic data obtained from six annually laminated (varved) lake sediment sequences and one non-laminated sediment sequence. The six independent varve chronologies were combined to form a timescale, which

The coarse to fine search methodology is frequently applied to a wide variety of problems in computer vision. In this paper it is shown that this strategy can be used to enhance the recognition of on-line handwritten characters. Some explicit knowledge about the structure of a handwritten character can be obtained through a structural parameterization. The Frame Deformation Energy matching (FDE) m

Parasites and diseases constitute major evolutionary forces in many natural populations, and thus having an efficient immune defense to resist infections is crucial for many organisms. Properties of the immune response may also influence mate choice decisions in many animals. Theory predicts several advantages for females when choosing males with superior immune systems. These benefits can be both

Liquid-crystalline cubic-phase nanoparticles (CPNPs) (known as Cubosome particles), based on the lipid glycerol monooleate and stabilized by the nonionic block copolymer Pluronic F-127, interact with supported model membranes consisting of dioleoylphosphatidylcholine (DOPC) in a complex and dynamic fashion. Neutron reflectivity measurements on the interaction of CPNPs with bilayers of different co

Members of the bone morphogenetic protein-1/tolloid (BMP-1/Tld) family of metalloproteinases, also known as procollagen C-proteinases (PCPs), control multiple biological events ( including matrix assembly, cross-linking, cell adhesion/migration and pattern formation) through enzymatic processing of several extracellular substrates. PCP activities on fibrillar procollagens can be stimulated by anot