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Novel techniques in molecular cytogenetics have radically improved the ability to characterize genetic changes in neoplastic cells. In parallel, a rapid development in high-throughput genomics has resulted in detailed physical maps of the human genome. Combining these two fields, we have developed a method for the simultaneous visualization of several physically defined segments along a chromosome
We used a series of CO5BOLD hydrodynamical model atmospheres covering stellar objects from white dwarfs to red giants to derive theoretical estimates of the photometric and photocentric stellar variability in wavelength-integrated light across the Hertzsprung-Russell diagram. We validated our models against solar measurements from the SoHO/VIRGO instrument. Within our set of models we f
The A (1)Sigma(+) potential energy curve of AgH is studied by means of the second-order multistate multireference perturbation theory including the spin-orbit and relativistic effects. The anomalous behavior of the vibrational energy levels observed in experiment is reproduced well by theory. An analysis of the A (1)Sigma(+) wave function shows that at the internuclear distance of 4-6 Angstrom, a
Prostate-specific antigen (PSA) is a widely used marker for prostate cancer. In the literature, there are reports of nonprostatic expression of PSA that potentially can affect early diagnosis. However, the results are scattered and inconclusive, which motivated us to conduct a more comprehensive study of the tissue distribution of PSA and the closely related protein human glandular kallikrein 2 (h
Mass, heat and momentum transport processes are coupled with catalytic chemical reactions in a methane steam reforming duct. it is often found that endothermic and exothermic reactions in the ducts are strongly integrated by heat transfer from adjacent catalytic combustion ducts. In this paper, a three-dimensional calculation method is developed to simulate and analyze reforming reactions of metha
Supermacroporous monolithic polyacrylamide (pAAm)-based columns have been prepared by radical cryo-copolymerization (copolymerization in the moderately frozen system) of acrylamide with functional co-monomer, allyl glycidyl ether (AGE), and cross-linker NN'-methylene-bis-acrylamide (MBAAm) directly in glass columns (ID 10 mm). The monolithic columns have uniform supermacroporous sponge-like struct
Although there is a high degree of homology in the M2 transmembrane segments of alpha1 and beta1 subunits, subunit-specific effects were observed in alpha1beta1 GABA(A) receptors expressed in Spodoptera frugipedra (Sf9) cells when the conserved 13' threonine residue in the M2 transmembrane region was mutated to alanine. When threonine 263 (13') was mutated to alanine in the beta1 subunit, high-aff
We present a theoretical study of vibrational and thermal effects on the dipole polarizability of methane and carbon tetrachloride. Using a fourth order Taylor expansion in rectilinear normal coordinates of the potential and property surfaces we solve the vibrational problem using vibrational structure theory, e.g., through vibrational self-consistent-field or vibrational configuration-interaction
Phosphatidylethanol (PEth), a direct ethanol metabolite, is detectable in blood for more than 2 weeks after sustained ethanol intake. Our aim was to assess the usefulness of PEth [comparing sensitivity, specificity and the area under the curve (AUC)] as compared with carbohydrate-deficient transferrin (CDT), gamma-glutamyl transpeptidase (GGT) and mean corpuscular volume (MCV), calculating the res
The proposed adaptive frequency domain finite element (FE) model makes it possible to simulate wave propagation in a pavement structure over a wide frequency range using a normal size computer. The geometry and mesh size are optimized and regenerated for each frequency using a simple script in a commercial FE software package. Results from the FE model agree well with normal mode dispersion curves
We study exciton migration in low molecular weight poly[3-(2,5-dioctylphenyl)thiophene] in dilute solution by means of ultrafast spectroscopy and Monte Carlo simulations of resonance energy transfer using the line-dipole Forster approach. The model includes the build-up of polymer chains, site-selective exciton generation, and diffusion through incoherent energy transfer. Time-resolved, ensemble-a
OBJECTIVE: To investigate the ability of statins to activate the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-gamma) in primary human monocytes in culture. MATERIALS AND METHODS: Human peripheral monocytes were incubated with atorvastatin (0.1-10 micromol/1) for up to 24 hours. PPAR-gamma expression was analysed by electrophoretic mobility shift assay. Pro-inflammatory c
Human islet amyloid polypeptide (hIAPP), a pancreatic islet protein of 37 amino acids, is the main component of islet amyloid, seen at autopsy in patients with type 2 diabetes mellitus (DM2). To investigate the roles of hIAPP and islet amyloid in DM2, we generated transgenic mice expressing hIAPP in their islet beta cells. In this study, we found that after a long-term, high-fat diet challenge isl
Genetic fine mapping can be performed by exploiting the notion that haplotypes that are structurally similar in the neighbourhood of a disease predisposing locus are more likely to harbour the same susceptibility allele. Within the framework of Generalized Linear Mixed Models this can be formalized using spatial smoothing models, i.e. inducing a covariance structure for the haplotype risk paramete
