We used site-directed mutagenesis to alter the specificity of human cystatin C, an inhibitor with a broad reactivity against cysteine proteinases. Nine cystatin C variants containing amino acid substitutions in the N-terminal (L9W, V10W, V10F and V10R) and/or the C-terminal (W106G) enzyme-binding regions were designed and produced in Escherichia coli. It was discovered that the inhibition profile

Man kan identifiera fem stora kundsegment på energimarknaden nämligen hushåll i småhuslägenheter, hushåll i flerbostadshusen lägenheter, fastighetsägare till flerbostadshus, unga hushåll samt medelålders och äldre hushåll. Jämfört med energiföretagens andra kundsegment inom exempelvis industri, handel, service eller myndigheter är hushållssegmenten oorganiserade. De innehåller många hushåll med be

Before 1990 environmental reporting in Sweden was a marginal and unsystematic activity undertaken by a small number of large companies in high-profile industries. The 1990's have witnessed a dramatic increase in environmental reporting practice, and today a large number of companies in almost all industries annually report considerable amounts of environmental information. Increasingly, companies

The present results demonstrate that chronic nicotine treatment can in part protect against mechanically-induced and neurotoxin-induced degeneration of nigrostriatal DA neurons. These results indicate that in sufficient doses chronic treatment with nicotine may be considered in the pharmacological treatment of Parkinson's disease. It remains to be demonstrated whether these protective actions can

The plant enzyme sucrose synthase (SuSy; EC 2.4.1.13) catalyzes the reversible conversion of sucrose and UDP into UDP-glucose (UDP-Glc) and fructose. The enzyme exists in different isoforms and is both located in the cytosol, membrane-bound and associated to the actin cytoskeleton. We here investigate sucrose synthase from tobacco (Nicotiana tabacum L.) BY-2 heterotrophic cell suspensions. Two dif

Objective-In clinical islet transplantation, the instant blood-mediated inflammatory reaction (IBMIR) is a major factor contributing to the poor initial engraftment of the islets. This reaction is triggered by tissue factor and monocyte chemoattractant protein (MCP)-1, expressed by the transplanted pancreatic islets when the islets come in contact with blood in the portal vein. All currently ident

This paper argues that there is a norm in the publication of quantitative management research that accepted hypotheses are conceived of as good hypotheses. This has far-reaching implications for quantitative research in that, in the worst case scenario, this norm encourages commonsensical research questions and manipulation of data in order for an article to be published. Naturally, such behavior

Temporal compressions of optical pulses and pulse trains have been performed by the photon-echo process in Tm-doped YAG at 793 nm. Single-pulse temporal compression by almost a. factor of 500 from 10 mu s to 22 ns and pulse train compression by a factor of 14 from 5 mu s to 350 ns with a high-speed frequency-tunable external-cavity diode laser are demonstrated. It is suspected that significantly h

The effects of two different prevention programmes on: (1) reported neck, shoulder and back pain, (2) perceived physical exertion at work and perceived work-related psychosocial factors, were evaluated by questionnaires after 12 and 18 months. Female nursing aides and assistant nurses (n = 282) working in the home-care services, were randomly assigned to one of three groups for: (1) individually d

In aqueous solutions hydrophobically modified polyethylene glycol (HM-PEG) forms a transient polymer network held together by intermolecular hydrophobic associations. In the present investigation we have used NMR-diffusometry to study how the addition of methylated alpha-cyclodextrin (M-alpha-CD) influences the polymer network. The addit on of M-alpha-CD resulted in an increased mean self-diffusio

The mol-ecule of the title compound, C14H14S, lies on a crystallographic twofold axis running through the central S atom, and hence mol-ecules have exact C-2 mol-ecular symmetry. Density-functional theory calculations reveal that the energy-optimized mol-ecular structure in the gas phase is close to that observed in the crystal structure. The crystal packing is dominated by double chains running a

Popular Abstract in Swedish Det har traditionellt sett varit mycket svårt att genomföra förbränningsstudier i motorer, men i och med laserns introduktion har nya möjligheter öppnats. Under de senaste 20 åren har lasern blivit ett allt viktigare hjälpmedel, och ett stort antal laser baserade mätmetoder har utvecklats. Det är nu möjligt att mäta temperaturer, ämneskoncentrationer, hastighetsfält ochMulti-dimensional, laser-based measurements of quantities important for obtaining a better understanding of combustion processes, particularly of temperature and of species distributions, were performed using laser-induced fluorescence (LIF). The main purpose of the temperature experiments was to investigate the potential of employing a two-line atomic fluorescence technique (TLAF), using indium a

Earlier light microscopic, immunocytochemical and morphometric investigations indicate that noxious substances transported with the vasogenic edema from hemispheric infarcts influence the character, timing and extent of the secondary thalamic lesions. The object of the present study was to analyze the ultrastructure of the secondary damage and the cytolytic nerve cell change which ensues in the th

BACKGROUND: Hereditary non-polyposis colorectal cancer (HNPCC) (Lynch cancer family syndrome I (LCFS1) and II (LCFS2)) is one of the most common hereditary cancer disorders. HNPCC results from dominantly inherited germline mutations in mismatch repair (MMR) genes, leading to genomic instability and cancer. No predictive physical signs of HNPCC are available to date. AIMS: Increased complexity in t

Popular Abstract in Swedish De flesta primärar hjärntumörer utgår ifrån astrocyter, oligodendrocyter och ependymala celler, som är hjärnvävnadens egna celler. Cirka hälften av dessa tumörer ingår i en grupp där tumörtypen heter gliom. Glioblastoma multiforme (GBM) är den mest elakartade tumören inom denna grupp och också den vanligast förekommande.Trots stora forskningssatsningar är behandlingsstrGlioblastoma multiforme (GBM) is the most malignant and also the most common form of brain tumour in humans. The overall aim of this thesis was to investigate morphological and diagnostics features in human GBM that may be of importance for prognosis and treatment. Immunohistochemically stained tissue sections have been microscopically analysed regarding immuno-reactive cells, cell proliferation,

Background: Loss of chromosome 11q defines a subset of high-stage aggressive neuroblastomas. Deletions are typically large and mapping efforts have thus far not lead to a well defined consensus region, which hampers the identification of positional candidate tumour suppressor genes. In a previous study, functional evidence for a neuroblastoma suppressor gene on chromosome 11 was obtained through m