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Rap1 is the main protein that binds double-stranded telomeric DNA in Saccharomyces cerevisiae. Examination of the telomere functions of Rap1 is complicated by the fact that it also acts as a transcriptional regulator of hundreds of genes and is encoded by an essential gene. In this study, we disrupt Rap1 telomere association by expressing a mutant telomerase RNA subunit (tlc1-tm) that introduces m
Cold region ecosystems store vast amounts of soil organic carbon (C), which upon warming and decomposition can affect the C balance and potentially change these ecosystems from C sinks to carbon dioxide (CO2) sources. We quantified the decadal year-round CO2 flux from an alpine steppe-ecosystem on the Tibetan Plateau using eddy covariance and automatic chamber approaches during a period of signifi
Graphene pn-junctions offer a rich portfolio of intriguing physical phenomena. They stand as the potential building blocks for a broad spectrum of future technologies, ranging from electronic lenses analogous to metamaterials in optics, to high-performance photodetectors important for a variety of optoelectronic applications. The production of graphene pn-junctions and their precise structuring at
Multi-pass cells (MPCs) have emerged as very attractive tools for spectral broadening and post-compression applications. We discuss pulse energy limitations of standard MPCs considering basic geometrical scaling principles and introduce a novel energy scaling method using a MPC arranged in a bow tie geometry. Employing nonlinear pulse propagation simulations, we numerically demonstrate the compres
Eyeblink conditioning is the most popular paradigm for studying classical conditioning in humans. But the fact that eyelids are under voluntary control means it is ultimately impossible to ascertain whether a blink response is ‘conditioned’ or a timed ‘voluntary’ blink response. In contrast, the pupillary response is an autonomic response, not under voluntary control. By conditioning the pupillary
Attosecond pulses, produced through high-order harmonic generation in gases, have been successfully used for observing ultrafast, subfemtosecond electron dynamics in atoms, molecules and solid state systems. Today's typical attosecond sources, however, are often impaired by their low repetition rate and the resulting insufficient statistics, especially when the number of detectable events per shot
Intrinsically disordered proteins (IDPs) are proteins that, in comparison with globular/structured proteins, lack a distinct tertiary structure. Here, we use the model IDP, Histatin 5, for studying its dynamical properties under self-crowding conditions with quasi-elastic neutron scattering in combination with full atomistic molecular dynamics (MD) simulations. The aim is to determine the effects
Understanding the electronic structure and chemical bonding of transition metal complexes is important for improving the function of molecular photosensitizers and catalysts. We have utilized X-ray absorption spectroscopy (XAS) and resonant inelastic X-ray scattering (RIXS) at the Fe L3 edge to investigate the electronic structure of two Fe N-heterocyclic carbene complexes with similar chemical st
Sweden needs affordable housing solutions to counteract segregation and isolation whilst promoting resilient cities. The COVID-19 pandemic has changed the conditions for being and sharing with others. The aim of the article is to explore residents practices of inhabiting, sharing and being involved in existing collaborative housing during the pandemic. The article conceptualizes collaborative hous
Production of 5-hydroxymethylfurfural from sugar beet molasses as feed-stock should in theory be a straight-forward process: first, hydrolysis of sucrose, followed by dehydration of fructose, but is in reality challenging. The enzyme-catalysed sucrose hydrolysis is inhibited by impurities and the dehydration of fructose has low conversions. In this study, pretreatment of crude molasses through pur
For the 20-year anniversary of ephemera journal, we have invited eight collectives engaged in alternatives to mainstream publishing to tell us about the initiatives they are part of. The task of ephemera, in turn, has been to bring them together. In this forum the collectives introduce what they do, articulating their political commitments and organising principles, whilst not shying away from the
Computational fluid dynamics (CFD) modeling may aid in planning of invasive interventions in Fontan patients. Clinical application of current CFD techniques is however limited by complexity and long computation times. Therefore, we validated a "lean" CFD method to magnetic resonance imaging (MRI) and an "established" CFD method, ultimately aiming to reduce complexity to enable predictive CFD durin
ObjectiveTo report trends and characteristics of post-biopsy infections, with regard to etiology and resistance patterns, in a large unique cohort from a single-centre using the same antibiotic prophylactic regimens during a 15-year period.MethodsThis is an observational cross sectional cohort study, including all patients who underwent transrectal prostate biopsy (TR PBx) guided by ultrasound for
An energy transition is unfolding in Canada and across the world. During this transition, countries are facing increasing demands for their energy systems to address economic, social, and environmental considerations, including providing affordable and reliable energy, reducing inequality, and producing fewer environmental impacts. First, we identify key themes from the academic literature related
We measured 135 cross sections of residual nuclei produced in fragmentation reactions of 12C, 14N, and 13−16,20,22O projectiles impinging on a carbon target at kinetic energies of near 400A MeV, most of them for the first time, with the R 3 B/LAND setup at the GSI facility in Darmstadt (Germany). The use of this state-of-the-art experimental setup in combination with the inverse kinematics techniq
This article analyses the role that ‘ordre public’ and morality exceptions can play in the granting of patents on inventions in the field of human germline editing and the consequences of this policy option. In order to provide the context for such an analysis, the article will, first, provide an overview of the current patent landscape for relevant genome editing technologies, drawing attention t
Crystallography is an invaluable tool in materials science, solid state physics and protein science. Understanding crystallography requires grasping the powerful but abstract concept of reciprocal space. Here a simple but insightful experiment using a laser pointer and Au microdisc arrays to explore and illustrate Bragg diffraction and reciprocal space is demonstrated. The Au microdisc arrays were
