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On-line monitoring of chromatographic procedures involving biomolecules is usually restricted to UV registration and determination of the pH or conductivity of the mobile phase. A more specific monitoring of proteins, for instance direct identification of a special enzyme, would greatly facilitate and speed up purification. We have found that flow calorimetry, performed with an enzyme thermistor i
Magnetic aqueous two-phase separation is a new technique to speed up the separation of aqueous two-phase systems (Anal. Biochem. 1987, 167, 331–339). It is based on the addition of magnetically susceptible material (e.g. 1-μ iron oxide particles) which induces rapid phase separation when a mixed system is placed in a magnetic field. The technique has been applied to a number of two-phase systems.
The effects of peptidolysis during early cheese ripening were studied in a low-fat (10% fat), round-eyed, semi-hard cheese using gel permeation chromatography and determinations of N content in cheese extracts. Addition of a heat-treated culture of Lactobacillus helveticus enhanced the peptidolytic activity even during the first weeks of cheese ripening. An aminopeptidase was found to have the nec
The reversal of the hydrolytic activity of α-mannosidase from jack bean has been studied in high concentrations of mannose. From an initial concentration of 85% mannose (w/w), a maximum yield of 70% (w/w) di- and oligosaccharides was obtained. The products were separated by gel chromatography and high-performance liquid chromatography, and characterized by gas-liquid chromatographymass spectrometr
β-d-Galp-(1→3)-d-GalNAc (1) was synthesised from lactose and GalNAc on a mmolar scale by transgalactosylation using β-d-galactosidase from bovine testes. The large proportions of unwanted oligosaccharides in the product mixture were removed by treatment with β-d-galactosidase from E. coli, which left 1, monosaccharides, and a small proportion of trisaccharides. Carbon-Celite chromatography then ga
This work employs ambient pressure X-ray photoelectron spectroscopy (APXPS) to delve into the atomic and electronic transformations of a core-shell Ni@NiO/NiCO3 photocatalyst - a model system for visible light active plasmonic photocatalysts used in water splitting for hydrogen production. This catalyst exhibits reversible structural and electronic changes in response to water vapor and solar simu
Galβ1-3GlcNAc (1) and Galβ1-3GlcNAcβ-SEt (2) were synthesized on a 100 mg scale by the transgalactosylation reaction of bovine testes β-galactosidase with lactose as donor andN-acetylglucosamine and GlcNAcβ-SEt as acceptors. In both cases the product mixtures contained unwanted isomers and were treated with β-galactosidase from Escherichia coli which has a different specificity, under conditions f
IntroductionPrevious studies show that approximately 40% of hospital readmissions in older adults are medication-related and that about 70% of these could be prevented. A risk assessment tool aiming to identify older adults at increased risk of medication-related readmission could help maximize the impact of implemented preventive measures and save healthcare resources. In this study we made furth
This entry offers a short description of basic research questions, different theories, and topics in lexical semantic research. At the beginning, it states five fundamental questions that all lexical semantics theories need to attend to in descriptions and explanations of their observations and findings. A brief presentation of past and present theoretical approaches on lexical semantics follows,
This special issue of the journal Urban Matters on “Intermedial Interventions in the City” explores the complexity of urban media life by adressing such questions as: how do intermedial processes shape, conceptualise, and otherwise intervene in the city? How do different kinds of media combinations produce and contest urban space? How are intermedial performances integrated into the social and pol
This study explores the integration of volunteers within Sweden’s public sector, specifically in disability services, probation services, and social services. In a study of public documents, it examines the differing logics of professional and voluntary social work, noting three key distinctions: practical preconditions, qualifications, and tasks. Professionals are paid employees with formal educa
Framtidens möten handlar om nya modeller för affärer, interaktion och kommunikation inom mötesindustrin. Rapporten undersöker hur pandemin påverkat mötesindustrin och om krisen fört med sig nya möjligheter. Fokus ligger på förändringar av branschens värdeerbjudande, hur gäster i fysiska, hybrida och digitala möten och konferenser interagerar och vilken roll värdskap och gästfrihet spelar i dessa. The Future of Meetings is about new business models, interaction, and communication in the meetings industry. The report examines how the pandemic has affected it and whether the crisis has brought new opportunities.It focuses on changes in the industry's value proposition, how guests in physical, hybrid and digital meetings and conferences interact and the role of hosting and hospitality in these
I projektet MDSEG (Myndighetsdriven Datadelning och Samverkan för EffektivareGodstransporter) strävar vi efter att främja ökad effektivitet inom lokal och regionalgodstransport genom datadelning och samarbete mellan olika aktörer. Vårtövergripande mål är att bidra till optimering av rutter för transporter och samordningav logistiken för att minska klimatpåverkan och öka effektiviteten. Vi har geno
Dick Harrisson undviker dendrokronologiska dateringar av Lunds äldsta gravar då de inte passar in i hans historiska modell. Som en parallell presenteras de dendrokronologiskt daterade pålar som spärrade Norrström omkring år 1000 vilka stockholmshistorikerna på sin tid inte accepterade.
Under andra världskriget gavs skolan en viktig pedagogisk uppgift för att stärka den ”andliga” beredskapen. Kunskaper ansågs förvisso som viktiga, men av större betydelse var en fostran till självständighet, handlingskraft, fosterlandskärlek och samfundsanda. För att stärka försvarsviljan skulle ungdomar också ges förståelse för vad det innebar att vara medborgare i ett fritt och demokratiskt samh
A previous systematic review has shown associations between exposure to high temperatures and negative birth outcomes. To date, a scoping review for heat indices and their use to measure effects of heat on maternal and perinatal health has not been considered.Objectives To provide a scoping review on heat stress and indices for those interested in the epidemiology and working in extreme heat and m
The study of Paul in the writings of “Hippolytus of Rome” is faced with some particularly difficult methodological challenges. In this chapter, therefore, I wish to do three things. First, I will introduce some of the fundamental problems in Hippolytan research, especially as they relate to the issues of authorship and the nature of the Hippolytan corpus. Second, to mitigate these problems for the
