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Hormonal regulation of sex pheromone biosynthesis in the turnip moth, Agrotis segetum

Pheromone production in the female turnip moth, Agrotis segetum, is under the control of a brain factor. This factor was demonstrated to be a proteinaceous substance termed pheromone biosynthesis activating neuropeptide‐like substance (PBAN‐like substance). The sex pheromone of Swedish A. segetum includes (Z)‐5‐decenyl acetate, (Z)‐7‐dodecenyl acetate, and (Z)‐9‐tetradecenyl acetate as major compo

Substrate specificity of acetyltransferase and reductase enzyme systems used in pheromone biosynthesis by Asian corn borer, Ostrinia furnacalis

The substrate specificity of the acetyltransferase and the reductase enzyme systems used by Ostrinia furnacalis (Lepidoptera: Pyralidae) in pheromone biosynthesis was studied in vivo by topical application of precursors to pheromone glands. Each of the tetradecenols, varying in double bond position (from 7 to 13) and geometry of the double bond, was converted to the corresponding acetate by the ac

Electrophysiological and behavioural evidence for a fourth sex pheromone component in the turnip moth, Agrotis segetum

Abstract. In addition to the pheromone components (Z)‐5‐decenyl, (Z)‐7‐dodecenyl and (Z)‐9‐tetradecenyl acetate (Z5‐10:OAc, Z7‐12:OAc and Z9‐14:OAc), it has previously been shown that the sex pheromone gland of the turnip moth, Agrotis segetum (Lepidoptera: Noctuidae Schiff) contains 10:OAc, 12:OAc, Z5‐12:OAc, Z9‐12:OAc, 11–12:OAc, Z5‐14:OAc, Z7‐14:OAc and Z11‐16:OAc. To find out whether any of th

Identification of a novel moth sex pheromone in Eriocrania cicatricella (Zett.) (Lepidoptera : Eriocraniidae) and its phylogenetic implications

Extracts from different body parts of adult female Eriocrania cicatricella (Zett.) were tested for electrophysiological activity on conspecific male antennae. Extracts from the Vth abdominal segment, containing a pair of exocrine glands, elicited the largest electroantennographic response when compared to extracts of other body parts. Female extracts were analyzed by gas chromatography with simult

A sex pheromone component novel to the Geometridae identified from Epirrita autumnata

(Z,Z,Z)‐1, 3, 6, 9‐Heneicosatetraene was identified as a sex pheromone component of Epirrita autumnata (Borkhausen), (Lepidoptera: Geometridae). Gas chromatography coupled with electroantennographic detection revealed one active peak‐from female pheromone gland extracts. The chemical identification of the compound was based on gas chromatography‐mass spectrometry, chemical micro‐reactions and unam

Control of sex pheromone biosynthesis in the moth Mamestra brassicae by the pheromone biosynthesis activating neuropeptide

The physiological route for the action of the pheromone biosynthesis activating neuropeptide (PBAN) was determined in Mamestra brassicae (L.) (Lepidoptera:Noctuidae). Removal of the ventral nerve cord including the terminal abdominal ganglia did not affect PBAN stimulation of pheromone production and the biogenic amine octopamine did not stimulate pheromone production in isolated abdomens. PBAN-li

Pheromone differences between sibling taxa Diachrysia chrysitis (linnaeus, 1758) and D. tutti (Kostrowicki, 1961) (Lepidoptera Noctuidae)

The noctuid sibling taxa Diachrysia chrysitis s. str. and D. tutti, of yet uncertain taxonomic status, have previously been shown to possess differences in morphology and to be attracted to different mixtures of the two presumed pheromone components (Z)-5-decenyl acetate and (Z)-7-decenyl acetate. Typical D. tutti males (clearly broken forewing marking) are known to respond to a 2: 100 mixture of

Pheromonal Production of and Response to Optically Active Epoxydienes in Some Ceometrid Moths (Lepidoptera: Geometridae)

In, pheromone extracts of calling female Chiasma clathrata, L. (Lepidoptera: Geometridae), a defoliator pest of alfalfa, (Z,Z,Z)-3,6,9–heptadecatriene and (Z,Z)-6,9–cis-3,4–epoxyhepta-decadiene was identified. Chiral gas chromatography using a modified cyclodextrin and synthetic reference samples proved the natural epoxide to show (3 R.4 S)-configuration. In field trapping tests, only the pure (3/

Pheromonal secretions from glands on the 5th abdominal sternite of hydropsychid and rhyacophilid caddisflies (Trichoptera)

Extracts of different body parts of adult Trichoptera were tested for electrophysiological activity. Extracts of the IVth and Vth abdominal sternites of female Hydropsyche angustipennis, Rhyacophila nubila, and R. fasciata, containing a paired exocrine gland, elicited significant electroan-tennographic responses when tested on conspecific male antennae. The paired gland occurs also in males of all

Moth pheromone genetics and evolution

Argues that there is no reason to dismiss species recognition as a possible cause of evolutionary change in moth sex pheromones. Selection for species recognition cannot explain all of the diversity in sex pheromones and the data supporting this contention are weak, but the alternative causes suggested, invoking mate choice between conspecifics as the mechanism of sexual selection, has no empirica

Species discrimination in five species of winter-flying geometrids (Lepidoptera) based on chirality of semiochemicals and flight season

Enantiomer separation of (6 Z,9 Z)-cis-3,4-epoxynonadecadiene and (3 Z,9 Z)-cis-6,7-epoxynonadecadiene could be achieved using chiral high-resolution gas chromatography and a cyclodextrin-bond column. (3 Z,9 Z)-(6 R,7 S)-Epoxynonadecadiene was identified from ovipositor extracts of Colotois pen-Naria, while in Erannis defoliaria the 6 S,7 R-enantiomer was found. In field trapping tests pure synthe

Functional specialization of olfactory glomeruli in a moth

The specific function of the glomerular structures present in the antennal lobes or olfactory bulbs of organisms ranging from insects to humans has been obscure because of limitations in neuronal marking methods. By tracing individual neurons in the moth Agrotis segetum, it was determined that physiologically distinct types of pheromone receptor neurons project axons to different regions of the ma

Attraction of male turnip moths Agrotis segetum (Lepidoptera : Noctuidae) to sex pheromone components and their mixtures at 11 sites in Europe, Asia, and Africa

Selected combinations of (Z)-5-decenyl, (Z)-7-dodecenyl, and (Z)-9-tetradecenyl acetates, the pheromone components of the turnip moth AgrotisSegetum were tested for field attractancy at six, two, and three sites in Europe, Asia, and Africa, respectively. At all of the sites in Eurasia and in northern Africa the ternary mixture of the acetates captured most males, while at the sites south of the Sa

Identification and bioassay of sex pheromone components of carob moth, Ectomyelois ceratoniae (Zeller)

Three sex pheromone components of the carob moth were isolated and identified from the extract of female pheromone glands, using a variety of techniques including coupled gas chromatographic-electroantennographic recordings, coupled gas chromatographic-mass spectrometric analysis, microozonolysis, electroantennographic assays of monounsaturated standards, wind-tunnel bioassays, and field trials. T

Epoxyheptadecadienes Identified as Sex Pheromone Components of Tephrina arenacearia Hbn. (Lepidoptera Geometridae)

(Z, Z)-3, 9-cis-6, 7-Epoxyheptadecadiene, and (Z, Z)-6, 9-cis-3, 4-epoxyheptadecadiene were identified by gas chromatography/flame ionization detection-electroantennographic detection (GC/FID-EAD) and coupled gas chromatography-mass spectrometry (GC-MS) from abdominal tip extracts of Tephrina arenacearia (Lepidoptera: Geometridae), an alfalfa pest. In gas chromatography/flame ionization—single sen

Sex pheromones and their potential role in the evolution of reproductive isolation in small ermine moths (Yponomeutidae)

Sex pheromone communication in the nine European species of small ermine moths (Yponomeuta) is reviewed in regard to the potential role of pheromones in the speciation process. Six of the nine species studied (viz., Y. evonymellus, Y. cagnagellus, Y. padellus, Y. irrorellus, Y. plumbellus, and Y. vigintipunctatus) use a mixture of (E)-11-and (Z)-11-tetradecenyl acetate in different ratios as prima

Sex pheromone biosynthesis in the Asian corn borer Ostrinia furnacalis (II) : Biosynthesis of (E)‐ and (Z)‐12‐tetradecenyl acetate involves Δ14 desaturation

Sex pheromone biosynthesis in the Asian corn borer Ostrinia furnacalis was studied by topical application of deuterium labelled fatty acids to the pheromone gland. The incorporation of the labelled acids into pheromone components and precursors was determined by gas chromatography with flame ionization detection and mass spectrometry in the selected ion monitoring mode. The labelling experiments s

Behavioural and electrophysiological activity of unsaturated analogues of the pheromone tetradecyl acetate in the small ermine moth Yponomeuta rorellus

ABSTRACT In field‐trapping experiments the unsaturated analogues (E)‐6‐, (E)‐12‐, and (Z)‐12‐tetradecenyl acetate were as attractive to male Yponomeuta rorellus Latr. as the native pheromone component tetradecyl acetate. All four analogues attracted more males than virgin females did, whereas (Z)‐6‐, (E)‐11‐, (Z)‐10‐ and (Z)‐11‐tetradecenyl acetate were essentially non‐attractive. Addition of 1–30

Population variation and genetic control of pheromone communication systems in moths

The nature of variation in moth pheromone communication systems and its genetic control is critical for the evolution of these systems and for their role in mate‐finding and reproductive isolation. Significant additive genetic variance has been demonstrated in female pheromone production in monomorphic populations. However, corresponding variance in male pheromone response with respect to the blen