In vivo evaluation of electron mediators for the reduction of methemoglobin encapsulated in liposomes by using electron energies produced by red blood cell glycolysis
Previous studies clarified that the electron enriched NADH and NADPH by RBC glycolysis could be used in an extracellular chemical reaction, where electron energies are extracted by an electron mediator methylene blue (MB). The reduced form of MB permeates the phospholipid bilayer of liposomes and reduce encapsulated ferric methemoglobin (metHb) to ferrous hemoglobin. Moreover, in vitro screening s