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We here describe the methods for the isolation of distinct hematopoietic subpopulations, as defined by their immune phenotype by fluorescence-activated cell sorting, and how these cells can be analyzed even at a single-cell level for the gene expression of a number of transcription factors and other differentiation markers.
We analyze the hp-version of the streamline diffusion finite element method for the Vlasov–Fokker–Planck system. For this method we prove the stability estimates and derive sharp a priori error bounds in a stabilization parameter δ ~ min(h/p, h2/σ), with h denoting the mesh size of the finite element discretization in phase-space-time, p the spectral order of approximation, and σ the transport cro
The extraction procedure for analysis of the raffinose family oligosaccharides (RFOs) in blanched leguminous vine peas was optimized. For this purpose, 25 pea samples from different cultivars and of different maturity were chosen at random. The effect of blanching was also studied in four cultivars. The yield of oligosaccharides was almost double on average when extracted with 50% ethanol as compa
Preschoolers’ prosocial and aggressive behaviors were explored longitudinally, with a focus on the inferred underlying motives of these behaviors. Forty-four children (initially 22–40 months) were observed in naturalistic interactions with peers, during a two-month period for each of three consecutive years. Three categories of prosocial behavior (requested, altruistic, and non-altruistic) and thr
Objective. To provide recommendations on the use of disease-modifying agents in the management of multiple sclerosis (MS) and to ensure that treatment will be available to those patients who may benefit. Methods. An initial draft of the consensus statement was prepared by the Steering Committee and amended in the light of written comments from a group of MS specialists. At a subsequent workshop, t
A number of numerical algorithms have been developed for various special classes of DAEs. This paper describes a new variable step size, constraint preserving integrator for general non-linear fully implicit higher index DAEs. Numerical implementation issues are discussed. Numerical examples illustrate the effectiveness of the new method.
Reaction of the tetradentate ligand N-(2-hydroxybenzyl)-N, N-bis(2-pyridylmethyl) amine (L - OH) with MoO2Cl2 in methanol in the presence of NaOMe and PF6- results in the formation of [MoO2(L - O)] PF6. Similarly, the reaction of N-(2-mercaptobenzyl)- N,N-bis(2-pyridylmethyl) amine (L -SH) with MoO2(acac)(2) leads to the formation of [MoO2(L - S)](+). The dioxo-molybdenum complex [MoO2(L - O)](+)
Here we studied whether glial cell line-derived neurotrophic factor (GDNF), given as a single bolus injection before an intrastriatal 6-hydroxydopamine (6-OHDA) lesion, can protect the nigrostriatal dopamine neurons against the toxin-induced damage and preserve normal motor functions in the lesioned animals. GDNF or vehicle was injected in the striatum (25 microg), substantia nigra (25 microg) or
Long-term time series of hydrogen chloride (HCl) and chlorine nitrate (ClONO2) total column abundances has been retrieved from high spectral resolution ground-based solar absorption spectra recorded with infrared Fourier transform spectrometers at nine NDSC (Network for the Detection of Stratospheric Change) sites in both Northern and Southern Hemispheres. The data sets span up to 24 years and mos
SUMMARY Repetitive samples of three strains of the moujd Penicillium were subjected to pyrolysis-gas chromatography (Py-GC). From the chromatograms, 26 peak heights were used in a subsequent SIMCA pattern recognition analysis. This data analysis gives a marked improvement in the classification of the samples (100% correct, 85% unique) in comparison with the traditional analysis based on the averag
In some Wireless Sensor Network (WSN) deployments, nodes may be used for different purposes (sensing, data pre-processing, actuation, etc.). Even if such a network is organised into clusters, this creates heterogeneity in terms of energy capability, which needs to be managed and balanced in order to ensure proper sensing capability with maximised lifespan. In this paper, we present Routing based o
Wireless sensor networks are composed of a large number of sensor nodes with limited energy resources. Once deployed, the sensor nodes are usually inaccessible to the user, and thus replacement of the energy resource is not feasible. Hence, energy efficiency is a key design issue that needs to be enhanced in order to improve the life span of the entire network. Several routing protocols have been
We have carried out a comprehensive molecular mapping of PG-M/versican isoforms V0-V3 in adult human tissues and have specifically investigated how the expression of these isoforms is regulated in endothelial cells in vitro. A survey of 21 representative tissues highlighted a prevalence of V1 mRNA, demonstrated that the relative frequency of expression was V1>V2>V3greater than or equal toV2; and s
Central-place foraging insects such as desert ants of the genus Cataglyphis use both path integration and landmarks to navigate during foraging excursions. The use of landmark information and a celestial system of reference for nest location was investigated by training desert ants returning from an artificial feeder to find the nest at one of four alternative positions located asymmetrically insi
