A Probing Feeding Strategy for Escherichia coli Cultures
A strain-independent feeding strategy for fed-batch cultures of Escherichia coli is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then us
