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Ornithine decarboxylase and S-adenosylmethionine decarboxylase expression during the cell cycle of Chinese hamster ovary cells

Cells in mitosis were harvested from exponentially growing Chinese hamster ovary cells by the mitotic detachment technique. Immediately after harvesting, the mitotic cells were seeded in tissue culture flasks and incubated at 37 degrees C in a CO2 incubator. Care was taken not to perturb the progression of cells through the cell cycle. At every hour after seeding for 14 h, cells were collected for

A validated decision model for treating the anaemia of myelodysplastic syndromes with erythropoietin plus granulocyte colony-stimulating factor: significant effects on quality of life

We have published previously a prototype of a decision model for anaemic patients with myelodysplastic syndromes (MDS), in which transfusion need and serum erythropoietin (S-Epo) were used to define three groups with different probabilities of erythroid response to treatment with granulocyte colony-stimulating factor (G-CSF) + Epo. S-Epo less than or equal to 500 U/l and a transfusion need of < 2

Effect of meteorological exchange conditions on PM10 concentration

The short-term limit value for PM10 having become effective in January 2005 was exceeded in many regions within the EU in 2005 and 2006. Therefore, strategies, particularly with respect to traffic control, are discussed, which could cause reduced PM10 concentrations leading to a more effective compliance with the short-term PM10 limit value. These strategies are often justified on PM10 differences

Stiffness and diameter of the common carotid artery and abdominal aorta in women

Vascular disease is differentiated throughout the vascular regions with aorta more prone to dilatation and with peripheral arteries more prone to occlusive disease. In this study, we investigated the diameter and distensibility in the common carotid artery (CCA) and abdominal aorta (AO) in healthy females of varying ages to assess potential differences in the aging process. The diameter and pulsat

The protonation status of compound II in myoglobin, studied by a combination of experimental data and quantum chemical calculations: Quantum refinement

Treatment of met-myoglobin (Fe-III) with H2O2 gives rise to ferryl myoglobin, which is closely related to compound II in peroxidases. Experimental studies have given conflicting results for this species. In particular, crystallographic and extended x-ray absorption fine-structure data have shown either a short (similar to170 pm) or a longer (similar to190 pm) Fe-O bond, indicating either a double

Capillary-induced phase separation in binary and quasi-binary polymer solutions. A mean-field lattice study

A capillary-induced phase separation (CIPS) can arise in a polymer solution enclosed between two walls, provided the solution is close to phase separation. Here the CIPS phenomenon is investigated in binary and quasi-binary polymer solutions by a mean-field lattice theory. A quasi-binary polymer solution represents a binary polymer solution with a polydisperse polymer. The CIPS involves a new phas

The Names of the Dnieper Rapids in Chapter 9 of Constantine Porphyrogenitus’ De administrando imperio

New interpretations are given for [ν]Έσσουπῆ ‘watching’, ‘the guard’; Ἀειφὸρ ‘the ever-shallow rapids’; βαρουφόροϛ – βουλνηπράχ ‘the bare rapids; Λεάντι, βερούτζ ‘the violently rushing rapids’. Στρούκουν has a Greek ending and is not a dative plural. Ναστρεζή is a collective formation with a diminutive sense. Both names mean ‘the small rapids’. The reduced vowels are not manifested in these name

Quantitative and qualitative evaluation of plasma and urine alpha(1)-microglobulin in healthy donors and patients with different haemolytic disorders and haemochromatosis

Background: The haem-binding protein alpha(1)-microglobulin (alpha(1)m) is involved in protection against oxidative damage induced by extracellular haem/haemoglobin. A carboxy-terminally truncated form of alpha(1)m (t-alpha(1)m), formed by reactions with haemoglobin, degrades haem into a yellow-brown chromophore linked to the protein. The aim of this work was to investigate if t-alpha(1) in is pre

Is local distribution of the epiphytic lichen Lobaria pulmonaria limited by dispersal capacity or habitat quality?

By surveying and re-surveying 12 forest sites in southern Sweden for the epiphytic lichen Lobaria pulmonaria with a 9-year interval, and measuring tree-related habitat quality variables, we have investigated whether the local distribution of the lichen is limited by poor dispersal capacity or by habitat quality. Dispersal distances were measured indirectly as the distances between colonised trees

Synthesis and electron transfer studies of ruthenium-terpyridine-based dyads attached to nanostructured TiO2

A series of bis(terpyridine)Ru-II complexes have been prepared, where one of the terpyridines is functionalized in the 4'-position by a phosphonic or carboxylic acid group for attachment to TiO2. The other is functionalized, also in the 4'-position, by a potential electron donor. In complexes 1a, 3a, and 4a,b, this donor is tyrosine or hydrogen-bonded tyrosine, while in 2a it is carotenoic amide.

Identification of the Statistical Properties of the Cutting Tool Vibration in a Continuous Turning Operation - Correlation to Structural Properties

The cutting force dynamics in alloyed steel, stainless steel and cast iron have been investigated, although a direct measurement of the cutting forces could not be carried out. Usually, the response of the tool holder shank is measured by strain gauges and will be dependent on the stochastic character of the applied load and the structural dynamic properties of the tool holder. The characterisatio

A new species of Emersonella (Hymenoptera : Eulophidae), parasitoid on weevil eggs (Coleoptera : Curculionidae), from Costa Rica

Emersonella curculiovora sp. nov. ( Hymenoptera: Eulophidae) is described from the Central Valley in Costa Rica. The species is diagnosed and compared to other species in the genus. It was reared from eggs of an undescribed species of Camptocheirus ( Coleoptera: Curculionidae), from stems of Cinnamomum cinnamomifolium ( Lauraceae). The parasitoid is mainly solitary, but occasionally two individual

Combined diffusion weighting and CSF suppression in functional MRI.

In this study, EPI pulse sequences with diffusion weighting for reduction of contributions from large vessels and inversion pulses in order to minimize the effects of CSF pulsations and CSF partial volume effects were developed for BOLD contrast investigations in functional MR imaging. One inversion recovery echo-planar imaging (IR-EPI) pulse sequence and one IR-EPI with additional diffusion weigh

Children's cognitive appraisal of others' distressful and positive experiences

The study presents a theoretical model for understanding how children's cognitive processing of empathy-provoking information is linked to vicariously aroused feelings, and to prosocial and aggressive behaviour. Predictions from the model were tested in a cross-sectional sample of second-, fourth-, and sixth-graders (N = 175) and in a large sample of fourth-graders (N = 124). Two significant forms

Fermentation and bulking capacity of indigestible carbohydrates: the case of inulin and oligofructose.

The bulking index (i.e. the increase in faecal fresh weight in gram per gram indigestible carbohydrate ingested) with oligofructose and inulin is similar to that produced with other easily fermented fibres such as pectins and gums. Most studies in man have been performed at a level of 15 g/d and more investigations on lower intakes are needed to appoint the least intake for an effect. Concerning s

Telomeric sequence diversity within the genus Saccharomyces

Conservation of telomeric DNA repeat sequences has been found across evolutionarily diverse eukaryotes. Here we report on a marked telomeric sequence diversity within the budding yeast genus Saccharomyces. Cloning and sequencing of telomeric repeat units from S. castellii, S. dairensis, S. exiguus and S. kluyveri showed a length variation between 8 and 26 bp, as well as a distinct variation in the

Kinetics of coal desulphurization by Acidianus brierleyi

Acidianus brierleyi was demonstrated to remove pyritic sulphur from coal. A. brierleyi was also found capable of catalyzing the removal of what is normally reported as organic sulphur from coal 171US34. A kinetic analysis was performed by assuming a first order reaction. The first order kinetics allowed a comparison with literature data for Thiobacillus ferrooxidans. Also, formation of jarosite wa