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We describe a general approach to rapidly obtain the DNA sequence encoding the variable region of any immunoglobulin chain using the polymerase chain reaction and a mixture of upstream primers corresponding to the leader sequence, and one downstream primer designed from the conserved nucleotide sequence of the constant region. The approach was applied to five different hybridomas producing human m
Objective: To investigate gene expression of thyrostimulin in orbital and thyroid tissue from patients with and without Graves' disease. Design: Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used for detection of thyrostimulin gene expression in intraorbital adipose tissue from patients with severe ophthalmopathy and thyroid healthy controls in addition to thyrostimulin ex
Abstract is not available
A 43-L column photobioreactor wash tested for the treatment of acetonitrile Using a symbiotic consortium consisting of a Chlorella sorokiniana strain and a Comamonas strain. Complete biodegradation of 1 g acetonitrile/l was achieved in 79 hours under continuous illumination at 500 mu E/m(2) s and 26 degrees C. When the photobioreactor was operated at 26 degrees C under a 14/10 hours light/dark ill
A general model for pressure drop calculations in siphons and riser tubes has been developed. The model takes into account different physical flow phenomena such as inlet pressure drop and pressure drop in the tube. For each phenomenon, we applied theory and established correlations to calculate the separate accelerational, frictional, gravitational, and centrifugal pressure drops. Model results a
Uttorkning av betong är ett ämnesområde som under lång tid varit i fokus. Genom åren har många skadefall kunnat kopplas till alltför fuktiga betonggolv. Det är inte betongen som tar skada utan vissa material som placerats i direkt anslutning till betongen. Exempel är kemisk nedbrytning av lim under plastmattor, vilket skapat kemiska emissioner som vandrat ut till inomhusluften, eller som lett till
While populism and populist politics have been the focus of a wide range of studies in the social sciences, few analyses of populist phenomena have moved beyond the level of simple description. This study has three immediate aims: to critique existing theories of populism, to develop a new, analytical view of populism, and apply this view comparatively to two cases. This new view of populism emer
Chromatofocusing of proteins in a microcolumn format (750 mum i.d.) was evaluated with slurry-packed Mono P anion-exchanger beads (10 pin bead size) as chromatographic matrix. Evaluation was performed with three standard proteins in terms of flow rate, Polybuffer 96-acetic acid, pH 6.0, concentration (eluent buffer strength), and detection limit. A column with the dimension 750 pin i.d. x 50 mm le
Abstract is not available
OBJECTIVE: Self-rated health (SRH) is increasingly attracting attention as a predictor of morbidity and mortality while its relation to impaired function has been given less momentum. The aim of this study was to assess the relation between SRH and the risk of being awarded a disability pension and premature death. METHODS: Five birth-year cohorts of middle-aged men were invited to a screening pro
Parmelia ernstiae Feuerer & A. Thell, recently described from northwestern Germany, is reported from one locality in central Skåne, southernmost Sweden.
A search for the single production of doubly-charged Higgs bosons H-+/-+/- in ep collisions is presented. The signal is searched for via the Higgs decays into a high mass pair of same charge leptons, one of them being an electron. The analysis uses up to 118 pb(-1) of ep data collected by the H1 experiment at HERA. No evidence for doubly-charged Higgs production is observed and mass dependent uppe
