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An alpha/beta barrel is predicted for the three-dimensional (3D) structure of Bacillus subtilis ferrochelatase. To arrive at this structure, the THREADER program was used to find possible homologous 3D structures and to predict the secondary structure for the ferrochelatase sequence. The secondary structure was fit by hand to the selected homologous 3D structure then the MODELLER program was used
The aim of this study was to evaluate some bioassays that are different in principle: cell counting, colony forming assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), sulforhodamine B (SRB), crystal violet, and alamarBlue, with respect to their ability to measure cisplatin-induced cell death of in vitro-cultivated squamous cell carcinoma of the head and neck (SCCHN). Cispl
The principal fate during litter decomposition and mineralization is rather well established only for a limited number of elements, mainly essential plant nutrients and a few potentially very toxic metals. Elements which occupy the majority of the positions in the periodical system seems almost unknown in this context. The objectives were, therefore to assess differences in the behavior of 58 mine
Efficient policy instruments for reducing the emission of greenhouse gases are essential for the realisation of a successful climate policy. General economic policy instruments have the potential for providing efficient emission reduction as they equalise marginal costs for emission reduction between different actors. They also provide incentives for a great variety of responses such as energy eff
Semenogelins I and II are major coagulum-forming proteins in semen, and they are secreted mainly by the seminal vesicles. These proteins bind Zn2+ and act as substrates for prostate-specific antigen and transglutaminase. A variant semenogelin I lacking 60 amino acids has been described that occurs in different populations with an allele frequency of 1%-3%. To better understand the function of the
A high-spin rotational band with 11 gamma -ray transitions has barn observed in Tc-91. The dynamical moment of inertia as well as the transition quadrupole moment of 8.1(-1.4)(+1.9) eb measured for this band show the characteristics of a superdeformed band. However, the shape is more elongated than in the neighbouring A = 80-90 superdeformed nuclei. Theoretical interpretations of the band within t
The effects of continuous and intermittent inhalation of trichloroethylene (TCE) were studied in male and female mice. Plasma butyrylcholinesterase (BuChE) activity, body, liver, kidney and spleen weights were measured. The liver was studied histologically and motor activity measured with doppler radar. Continuous TCE-exposure (37–300 p.p.m.) increased plasma BuChE activity in the males in a time
Young entrepreneurs establishing enterprises in Hanoi, Vietnam, are faced with an economic environment very different from that of their parents' generation, as doi moi (renovation) introduced in 1986 creates an increasingly capitalist market economy. Drawing on field studies in Hanoi we consider whether such entrepreneurs are able to take advantage of social capital networks - namely, bonding, br
OBJECTIVES: To estimate the total direct medical costs to society for patients with type 2 diabetes in Sweden and to investigate how different factors, for example diabetic late complications, affect costs. DESIGN: Cross-sectional data regarding health care utilization, clinical characteristics and quality of life, were collected at a single time-point. Data on resource use cover the 6-month perio
A comparison was made of 15 different techniques which are used in assessing soil microbial populations and/or biomasses. These include direct observations (fungal standing crop, fluorescein diacetate active mycelia, acridine orange stained bacteria), cultural methods (bacterial plate counts), physiological methods (total microbial, bacterial and fungal biomasses, O2-uptake), soil enzyme analyses
To ensure energy to meet needs for economic growth and sustainable development more emphasis should be given to energy efficiency, renewable energy and new technologies for both energy end-use and supply. One key technology is gasification of coal to produce liquid and gaseous fuels, and electricity. The overall conclusion is that there are plausible energy-technology strategies, well within reach
We report numerical simulations oil shrinkage in a fiber network when the fibers shrink more in their transverse to length direction than the axial direction. The effect of network density and anisotropic fiber properties on sheet shrinkage, elastic modulus and segment strain distributions is explored. The macroscopic free shrinkage follows a simple mathematical expression that is consistent with
Limit distributions for the greatest convex minorant and its derivative are considered for a general class of stochastic processes including partial sum processes and empirical processes, for independent, weakly dependent and long range dependent data. The results are applied to isotonic regression, isotonic regression after kernel smoothing, estimation of convex regression functions, and estimati
Fordonens körmönster, dvs hastighets- och accelerationsmönster påverkar mängden utsläpp i hög grad. I tidigare studier har parametern andel kraftig acceleration (a>1,5m/s2) visat sig ha en signifikant effekt framför allt för avgasutsläpp av NOx och HC men även för bränsleförbrukningen. Ett förarstöd som styr accelerationen kan därmed ha en positiv effekt för att minska emissioner och bränsleförbru
