Using three cytotoxicity assays, we have investigated the effect of the spermine analogue N1,N11-diethylnorspermine (DENSPM)on four human breast cancer cell lines with different known genetic lesions. Cells were seeded in 96 well plates and DENSPM was added 24 h later to give final concentrations from 0.1 to 100 microM. At 24, 48 and 72 h of treatment, the protein content was determined with a mod

The vitamin K-dependent and ubiquitously expressed 82 kDa glycoprotein product of the growth-arrest-specific-gene 6, Gas6, shows 44% sequence identity to the anticoagulant protein, protein S. Both proteins share further the same domain organization. A short loop region, 4 epidermal growth factor-like modules and an SHBG-like region follow the N-terminal Gla-domain, which confers membrane-binding c

STUDY DESIGN: Prospective randomized longitudinal clinical trial with matched controls. OBJECTIVES: To investigate the long-term effect of training on postural control and extremity function after an acute anterior cruciate ligament (ACL) injury. BACKGROUND: ACL injuries may cause severe problems with recurrent giving way of the knee and reduced functional capacity. The effect of an acute ACL inju

BACKGROUND: The Arg389 variant of the beta(1)-adrenergic receptor gene mediates a higher isoproterenol-stimulated adenylate cyclase activity than the Gly389 variant in vitro. We investigated whether the Arg389Gly or the Ser49Gly polymorphism is associated with hypertension in Scandinavians. Methods and Results-- A total of 292 unrelated, nondiabetic, hypertensive patients and 265 unrelated healthy

Quick-release and quick-stretch experiments have been performed on preparations of smooth muscle from rat portal vein and rabbit urinary bladder. The low equivalent mass of the isotonic lever (8 mg) implied that inertial oscillations were limited to the first 5-10 msec after the load step. The high time resolution achieved in this way enabled us to separate three components in the length response

Children’s target experiences (as recipients of prosocial peer acts and victims of peer aggression) were investigated for their concurrent and longitudinal associations with prosocial and aggressive behavior. Forty-four children (initially 22–40 months) were observed in naturalistic interactions with peers during a two-month period for each of three consecutive years. Results revealed no consisten

Methods that objectively assess the muscle fibre-type arrangement may improve the detection of fibre-type grouping, a diagnostic sign of a denervation and reinnervation process. To distinguish between a diseased and a normal muscle, there is a need for quantitative data on the fibre-type arrangement in healthy human muscles at different ages. In this study, cross-sections were prepared of whole au

Abstract is not available.

The influence of a small hydrophobic solute (octanoic acid) on the flux reduction and the flux recovery of two ultrafiltration membranes was studied in this investigation. The two membranes were made of polyether sulfone and regenerated cellulose, both with a nominal molecular weight cutoff of 20,000 Da. The concentration of the octanoic acid solution was found to have a significant influence on n

In this work it is shown that the power of the intermodulation noise of a wide-band amplifier with dynamic nonlinearities can be estimated by the total harmonic distortion (THD) with a sinusoid input signal of appropriate amplitude and frequency. The THD is, as opposed to the intermodulation noise, easy to measure and use as a design parameter.

Uptake capacity of organic nitrogen was studied in solution experiments on eight grasses and two forbs growing in acid soils with relatively high nitrogen mineralisation in southern Sweden. Uptake of a mixture of amino acids (alanine, glutamine, glycine), that varied between 1.6 and 6.3 mu mol g(-1) dw root h(-1), could not be explained by soil data from the species' field distributions (pH, total

The authors describe a patient who presented from birth on a severe involvement of connective tissues with pathological fractures, lack of auricular cartilage, hyperlaxity of fingers and cutis laxa with deep folds, all suggestive of derangements of collagen and elastin. Hypothermia at 24 hours of age should have already indicated the possibility of Menkes' syndrome. From the 3rd month on, the pati

Sleep disordered breathing (SDB) including obstructive and central sleep apnoea/ hypopnoea as well as periodic breathing (PB) is common and is believed to increase risk for mortality in patients with congestive heart failure (CHF). Mandibular advancement device (MAD) has widely been recommended for treatment of obstructive sleep apnoea but the method has never been investigated for treatment of SD

Background. Various outcomes have been described during pregnancy and among infants born to women after in vitro fertilisation (IVF) treatments. This mini-review summarises recent population-based Swedish studies about the short- and long-term effects of IVF on the infant and child, and also comments on disturbances of pregnancies and deliveries occurring after IVF. Methods. Data on women who had

The rat stomach is rich in endocrine cells. The acid-producing (oxyntic) mucosa contains ECL cells, A-like cells, and somatostatin (D) cells, and the antrum harbours gastrin (G) cells, enterochromaffin (EC) cells and D cells. Although chromogranin A (CgA) occurs in all these cells, its processing appears to differ from one cell type to another. Eleven antisera generated to different regions of rat