Designing a Multifunctional Chromatography Stationary Phase for (Bio)Product Purification via Green Chemistry
The multifunctional cryogels were synthesized by atom transfer radical polymerization (ATRP) using hemoglobin as a biocatalyst (ATRPase) and post-modified to purify hemoglobin and glycoprotein simultaneously. Meanwhile, the optimization of purified glycoprotein was also conducted by introducing epoxy group nanoparticles. The nanoparticle concentrated concanavalin A, the ligand, to adsorb glycoprot