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Aquaporin 2 (AQP2) acts as a water channel in the apical membrane of principal renal cells in the collecting ducts of the kidney. Upregulation is controlled by stimulation of these cells with vasopressin. Downregulation of the presence of AQP2 in the membrane occurs through ubiquitination and then sorting of the protein via the ESCRT-I, -II and –III complexes into the Multivesicular bodies (MVBs).
