Release of dog polymorphonuclear leukocyte cathepsin g, normally and in endotoxin and pancreatitic shock : Isolation and partial characterization of dog polymorphonuclear leukocyte cathepsin g
Dog polymorphonuclear leukocyte cathepsin G was isolated from a granule extract using a two-step procedure including affinity chromatography on aTrasylol-Sepharose gel and ion-exchange chromatography on a CM 52 column. 22 of the first 24 N-terminal amino acids were determined and showed 83% and 71% identity to those of human and rat cathepsin G, respectively. Total amino-acid composition demonstra