Non-invasive monitoring of protein adsorption and removal in a turbulent flow cell
A flow cell was designed for continuous non-invasive measurements of macromolecular adsorption and removal under well-defined flow conditions. The adsorption of the protein β-lactoglobulin to stainless steel surfaces and its subsequent detergent-induced removal were followed in the flow cell using in situ ellipsometry under both laminar and turbulent conditions. Factors varied include the cleaning