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A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets.

Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation tec

Evasive response to ultrasound by the crepuscular butterfly Manataria maculata

The crepuscular nymphalid butterfly Manataria maculata was studied in Monteverde cloud forest, Costa Rica, during the dry season reproductive diapause. M. maculata has ears in the form of Vogel's organs located near the base of the forewings. Its behaviour in response to bursts of ultrasonic pulses (26 kHz, 110 dB SPL at 1 m) was condition-dependent. At dusk and dawn the sound consistently elicite

Shot noise in tunneling through a single quantum dot

We investigate the noise properties of a zero-dimensional InAs quantum dot (QD) embedded in a GaAs-AlAs-GaAs tunneling structure. We observe an approximately linear dependence of the Fano factor and the current as a function of bias voltage. Both effects can be linked to the scanning of the three-dimensional emitter density of states by the QD. At the current step the shape of the Fano factor is m

Cretaceous palynofloras from southern Scandinavia

Popular Abstract in Swedish Avhandlingen omfattar studiet av fossila pollen och sporer (palynologi) från skånes krita (90-140 miljoner år gamla sediment). För ändamålet har tre borrkärnor provtagits; Höllviken 2, Norrevång 1 och Svedala 1, samt ytprover från överkritan på Bornholm. RESULTAT * Taxonomiska studier baserade på ca 100 arter (ca. 15 000 exemplar), har möjliggjort en stratigrafisk indeA palynological investigation of Cretaceous sequences from Höllviken 2, Svedala 1 and Norrevång 1 wells of Scania, Southern Sweden, and from the Arnager Greensand-Arnager Limestone transition on the island of Bornholm, Denmark, was conducted. Well preserved palynofloras were found in strata consisting of shaly mudstones, glauconitic sandstones (greensand) and limestone. A total of 98 palynomorph t

Jämviktsprovtagning

Jämviktsprovtagning genom membran är ett nytt koncept där porösa hålfibrer används för upprening, anrikning och mätning av polära organiska föreningar och metaller. Metoden kan användas såväl i laboratoriet som till fältprovtagning

Differential impairment of glucagon responses to hypoglycemia, neuroglycopenia, arginine, and carbachol in alloxan-diabetic mice.

To gain insight into the mechanisms responsible for the loss of the glucagon response to insulin-induced hypoglycemia in type 1 diabetes, glucagon responses to 4 different stimuli were examined over 3 months of diabetes in alloxan-treated mice. At 1, 6, and 12 weeks after alloxan (60 mg/kg), phloridzin (0.1 g/kg) was administered to overnight fasted diabetic mice to match the glucose levels of tho

Design och designprocess för en hållbar arbetsmiljö: en utvärdering av projektet Skåne i god form II. Ett samarbetsprojekt mellan LO-distriktet i Skåne, SVID och Svenska ESF-rådet

Utgångspunkten för projektet Skåne i god form II har varit de problem på arbetsmarknaden som kan relateras till arbetsmiljön och som resulterar i hög sjukfrånvaro och svårigheter att behålla och nyrekrytera arbetskraft. Projektet har syftat till att skapa möjligheter för de anställda att tillsammans med designer granska sin egen arbetsmiljö och de arbetsuppgifter man har och utarbeta förslag på hu

Hamilton's rule confronts ideal free habitat selection

If individuals occupy habitats in a way that maximizes their fitness, if they are free to occupy the habitats they choose and if fitness declines with population density, then their abundance across habitats should follow an ideal free distribution. But, if individuals are genetically related, this simple fitness-maximization mechanism breaks down. Habitat occupation should obey Hamiltons rule (na

The pursuit of heavy elements in the HgMn-type star Chi Lupi: Observations with the GHRS in the COSTAR ERA

Observations of the ultra-sharp-lined HgMn star chi Lupi made with the Goddard High Resolution Spectrograph after the Hubble Space Telescope First Servicing Mission display the effect of COSTAR corrective optics upon the spectral resolution of several observing modes. The resolution is restored to levels expected in the absence of the telescope spherical aberration for all observations made throug

Shadows of language in physics and cybernetics

In a large variety of disciplines, fundamental studies often straddle in self-referential situations, in need of relativization to language and complementaristic resolution. In such attempts, the languages at play are hardly visible themselves. Only shadows of language, somewhat characteristic of the various disciplines, become visible and tractable. We select two domains, quantum mechanics and cy

Contractions activate hormone-sensitive lipase in rat muscle by protein kinase C and mitogen-activated protein kinase

Intramuscular triacylglycerol is an important energy store and is also related to insulin resistance. The mobilization of fatty acids from this pool is probably regulated by hormone-sensitive lipase (HSL), which has recently been shown to exist in muscle and to be activated by both adrenaline and contractions. Adrenaline acts via cAMP-dependent protein kinase (PKA). The signalling mediating the ef

Capsular distance in the hip of the healthy child - normal values with sonography and MR imaging.

PURPOSE: To determine the normal values of the anterior and posterior capsular distances of the hip joint in healthy children by means of US, using MR imaging as reference, and to evaluate any possible correlation between age, length, weight and anterior capsular distance (ACD). MATERIAL AND METHODS: In our first study both hips in 14 healthy children (5-18 years old) were examined with US and MR

A fibroblast heparan sulphate proteoglycan with a 70 kDa core protein is linked to membrane phosphatidylinositol

Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence

Aerobic biodegradation of nonylphenol by cold-adapted soil bacteria.

Three strains capable of mineralizing nonylphenol as sole carbon source were isolated from a sample of contaminated soil and characterized as two Pseudomonas spp. and a Stenotrophomonas sp. The two Pseudomonas spp. expressed characteristics typical of psychrophiles growing optimally of 10 °C and capable of growing at 0 °C. The Stenotrophomonas sp. was more likely psychrotrophic because it had an o