Aspartylglucosaminidase (AGA, EC 3.5.1.26) is an essential enzyme in the degradation of asparagine-linked glycoproteins. In man, deficient activity of this enzyme leads to aspartylglucosaminuria (AGU), a recessively inherited lysosomal storage disease. Here we used affinity-purified polyclonal antibodies against the native AGA and its denatured subunits to establish the molecular structure and int

The Ohio State University rate of heat release apparatus was calibrated with two measurement systems, the standard compensated thermopile and an instrumentation for oxygen consumption measurements. Based on the calibration results the oxygen consumption technique was chosen when testing 13 different materials including wood-based materials, wallcoverings and plastics. The same set of materials has

This paper presents a manual that covers the necessary design steps for a basic ASIC design flow. It is shown how the manual writing process is organized such that each chapter covers a certain step in the design flow. The manual has been written especially with practicality in mind and has been successfully applied to undergraduate and postgraduate teaching

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Culturable and total bacterial counts, bacterial growth rate and tolerance to pH, as well as microbial biomass, were studied in two coniferous forest soils. The pH had been changed by addition of lime and wood-ash from 4.3-4.4 to 7.0 in one soil and from 3.9-4.4 to 6.1 in the other. Higher total microbial activities and higher bacterial growth rates, measured as soil respiration rate and thymidine

The differential absorption structure of the ozone spectrum between 250 and 330 nm has been investigated in order to determine the optimal wavelength region to be utilized for active differential optical absorption spectroscopy (DOAS) measurements. Considering aspects of atmospheric attenuation and interference from other species as well as the magnitude of the differential absorption cross sectio

Abstract What are the pedagogical impacts of internationalisation on the development of higher education in Europe? How can we proceed in this process and take a pedagogical stance on the issue? This theoretical paper is partly based on a series of empirical studies conducted by the author, investigating students’ and teachers’ experiences and understanding of an internationalised educational cont

We find good rate 2/3 and 3/4 convolutional codes for use with iterative decoding (turbo equalization) of coded intersymbol interference with AWGN. The method is based on the input-output bit error rate characteristic of the convolutional BCJR decoder module. An analysis leads to an equivalent minimum distance under the required intrinsic subtraction, which depends on free distance and an input-ou

Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. environment, foodstuffs, clinical, pharmaceutical, diagnosis, etc.). Recently, the immuno

This thesis examines the creative appropriation of Shakespeare in theory and practice. It is based on close readings of King Lear alongside three appropriations of the play in works of fiction by three North American women writers: Jane Smiley, Anne Tyler, and Margaret Atwood. The aim is to demonstrate how a ‘palimpsestic’ reading, i.e. a reading in which the reader oscillates between ‘pre-text’ a