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Proteins and peptides in bovine milk and colostrum were separated by two-dimensional gel electrophoresis (2-DE). The proteins were separated in the first dimension by isoelectric focusing in the pH ranges 4-7 and 6-11, and in the second dimension by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 12.5% T homogeneous gels. The peptides were separated in the pH range 3-10 in the first
OBJECTIVE: To investigate whether methylhexahydrophthalic acid (MHHP acid) in urine and plasma can be used as a biomarker for exposure to methylhexahydrophthalic anhydride (MHHPA). METHODS: MHHPA in air was sampled by Amberlite XAD-2 and analysed by gas chromatography (GC) with flame ionisation detection. MHHP acid in urine and plasma was analysed by GC with mass spectrometric detection. Workers o
Objective. We recently showed that plasma concentration of N-terminal atrial natriuretic peptide (Nt-proANP) is strongly directly related to salt sensitivity. The aims of the present study were to test (i) whether plasma concentration of N-terminal brain natriuretic peptide (Nt-proBNP) is related to salt sensitivity and (ii) whether Nt-proANP, as a marker of salt sensitivity, differs between type
Hepatitis C virus (HCV) genotypes, determined by polymerase chain reaction with type-specific primers, were studied in 5 already HCV-infected patients receiving kidneys from HCV-infected cadaver donors. Three patients were investigated retrospectively using stored pre- and posttransplantation sera and followed 18-28 months after transplantation. Two recipients with HCV genotype 2b infection had re
The aim of this study was to establish the influence of alpha-ketoglutarate (AKG), administered to pregnant sows from the 91(SI) d of pregnancy to farrowing, and then to piglets from birth to the 30(th) d of life, on lysozyme and ceruloplasmin activity, serum total protein content, and the WBC counts in blood of piglets, at the age of the 14(th) and 30(th) d of their postnatal life. The sows were
Inside-out submitochondrial particles from both potato (Solanum tuberosum L. cv. Bintje) tubers and pea (Pisum sativum L. cv. Oregon) leaves possess three distinct dehydrogenase activities: Complex I catalyzes the rotenone-sensitive oxidation of deamino-NADH, NDin(NADPH) catalyzes the rotenone-insensitive and Ca2+-dependent oxidation of NADPH and NDin(NADH) catalyzes the rotenone-insensitive and C
Dietary supplements, designed to fortify the antioxidant status of human metabolism, are intended to provide nutritional support against the aging process. The strategy is based on the observation that environmental and metabolic sources of oxidatively generated free radicals damage key macromolecules, such as DNA, that in turn alter physiologic processes, and in turn may contribute to aging and a
STUDY DESIGN: By implanting tantalum indicators percutaneously during application of pedicular screws, lumbosacral mobility could be studied with roentgen stereophotogrammetric analysis in seven patients having a diagnostic external fixation test. OBJECTIVES: To determine the mechanical effects on the segmental mobility during an external fixation test of the lumbar spine. SUMMARY OF BACKGROUND DA
Abstract is not available
PURPOSE: The frequency of activated protein C (APC) resistance, caused by factor V R506Q gene mutation and abnormal APC ratio, in patients with peripheral vascular diseases was analyzed. METHODS: All patients electively admitted to the vascular ward unit of our tertiary care academic medical center from January 1995 through October 1996 (n = 679) were prospectively analyzed using an APC-resistance
It was shown by Southern hybridization that cotton-top tamarin and common marmoset, New World monkeys, carry three or more genes encoding beta-microseminoprotein, also known as PSP94. In contrast, the genomes of Old World monkeys, as represented by rhesus macaque and sacred baboon, contain a single gene. Clones containing three different genes encoding beta-microseminoprotein were isolated from a
"Foreword: The durability of building materials, especially the corrosion of reinforcement in concrete structures, has been a main research area in Scandinavia during the last decades. Results from many laboratories in the Scandinavian countries, and outside of Scandinavia, indicate that there are certain relationships between the service life and different concrete parameters, such as the water/b
Children already have intimal thickenings occupying over half of the vascular lumen. Intimal thickening is one of the first steps in atherogenesis. Classical risk factors such as hypercholesterolaemia, genetic background and acute noxious factors play a role in its development. Genetic origin of the children from high-risk groups for coronary heart disease seems to be a determinant of the magnitud
Finite size effects oil cluster distributions are investigated within Ising and Fisher's Droplet Model. Fixed magnetization calculations suggest that, the introduction of a complement correction of the emitting system is a promising approach for finite size scalings.
The high level of protection required by personal protective clothing (PPC) severely impedes heat exchange by sweat evaporation. As a result work associated with wearing PPC, particularly in hot environments, implies considerable physiological strain and may render workers exhausted in a short time. Recent development of algorithms for describing the heat transfer, accounting for pumping and wind
This paper reports detailed abundance analyses for four extremelymetal-poor (XMP) giant stars with [Fe/H]<-3.8, based onhigh-resolution, high-S/N spectra from the ESO VLT (Kueyen/UVES) and LTEmodel atmosphere calculations. The derived [alpha /Fe] ratios in oursample exhibit a small dispersion, confirming previous findings in theliterature, i.e. a constant overabundance of the alpha -elements with
A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ion
