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The heterotrimeric basement membrane protein laminin-111 is essential for early mouse embryogenesis. Its beta1 and gamma1 chains are crucial for endoderm differentiation and for the formation of basement membranes, whereas alpha1 chain null mice only lack the extraembryonic Reichert's membrane. Nevertheless, mice deficient in the cell-binding alpha1 globular domains 4-5 (LG4-5) have a more severe
A (PCP)PdMe complex has been synthesized and structurally characterized, displaying a very long Pd-Me bond. It is reactive toward CO2 insertion, giving the corresponding acetate in quantitative yield. The methyl complex can be regenerated using ZnMe2, and catalytic carboxylation is possible in benzene.
Recombinant amorpha-4,11-diene synthase from Artemisia annua, expressed in Escherichia coli, was incubated with the deuterium-labeled farnesyl diphosphates, (1R)-[1-H-2]FPP, (1S)-[1-H-2]FPP, and [1,1-H-2(2)]FPP. GC-MS analysis of amorpha-4,11-diene formed from the deuterated FPPs shows that the deuterium atoms are retained in the product. Furthermore, analysis of the MS-spectra obtained with the d
Background. Several population-based studies have shown that self-perceived health is a powerful predictor of health outcomes. The extent to which self-perceived health is associated with personality characteristics is, however, largely unknown. We aimed to study the relationship between self-perceived health and personality among adults in the community. Method. Data were drawn from the Midlife D
Poorly oxygenated ( hypoxic) tumors are frequently more aggressive compared to corresponding tumors that are better oxygenated. Adaptation to hypoxia is primarily mediated by two closely related hypoxia inducible transcription factor complexes, HIF-1 and HIF-2, which become stabilized and activated at low oxygen levels. Whether HIF-1 and HIF-2 have different roles in tumorigenesis is an open quest
A major limitation to clinical stem cell-mediated gene therapy protocols is the low levels of engraftment by transduced progenitors. We report that CXCR4 overexpression on human CD34(+) progenitors using a lentiviral gene transfer technique helped navigate these cells to the murine bone marrow and spleen in response to stromal-derived factor 1 (SDF-1) signaling. Cells overexpressing CXCR4 exhibite
Screening of 29 strains of Neisseria gonorrhoeae revealed that 16/21 serum resistant strains and 0/8 serum sensitive strains bound C4bp. suggesting that C4bp binding to gonococci could contribute to serum resistance. C4bp bound to gonococci retained cofactor (C4b-degrading) function. Using allelic exchange to construct strains with hybrid Por1A/B molecules, we demonstrate that the N-terminal loop
It is important that the properties of a Virtual Reality (VR) model support its purpose in the specific context in which it is used. This study investigated how employees of a company experienced a VR model of their yet-to-be-built office building. We also compared the VR experience with the employees' experience of the completed building. The results showed that the employees felt that the VR mod
The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that i
Selective supercritical fluid extraction (SFE) at 40 °C, 120 bar and 60 min was utilised as a means to estimate the bioavailable fraction of PCBs to chironomid larvae in a naturally contaminated limnic sediment. This extraction methodology removed about 50% of the PCBs from the sediment. According to the equilibrium partitioning theory, organisms in that sediment should decrease their uptake from
This investigation describes the discovery of novel possible cerebrospinal fluid (CSF) biomarkers for frontotemporal dementia (FTD) using surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS). Sixteen clinically diagnosed FTD patients and 12 non-demented controls were included in the study. CSF was collected and analyzed for protein expression by SELDI-TOF
Respiratory sinus arrhythmia (RSA) was examined in relation to state and trait anxiety in healthy individuals. Time-frequency analyses of HR-power spectrum in the high frequency region (0.12-0.40 Hz), related to RSA, were examined in 43 women and 39 men. Based on median split, the participants were divided into high and low state and trait anxiety groups. The main result showed that high state anx
Allergic contact dermatitis is recognized as a public health problem and some major allergens have been subject to intervention aiming at lowering skin exposure. There is an obvious interest in evaluating the effect of such interventions. Population studies are difficult to perform and epidemiological studies based on clinical data from testing patients with contact dermatitis are common surrogate
We studied the effects of 5 mA bilateral or unilateral, bipolar or monopolar, galvanic stimulation on the horizontal vestibulo-ocular reflex (hVOR) in six normal subjects during 0.01, 0.05, 0.1, 0.5 and 1 Hz yaw rotations and in two subjects during high-acceleration, low-amplitude yaw head rotations (head impulses). Bipolar galvanic stimulation induced horizontal nystagmus in all subjects and an a
This study analyses the associations between sense of coherence and health and between stress and health in adolescents. In addition, this study tests whether a high sense of coherence could modify the health effects of stress. The study was a Danish cross-sectional analysis of 3258 adolescents from school classes in a national representative sample. The child, the main teacher and the school doct
Activated Lactococcus lactis beta-phosphoglucomutase (beta PGM) catalyzes the conversion of beta-D-glucose 1-phosphate (beta G1P) derived from maltose to beta-D-glucose 6-phosphate (G6P). Activation requires Mg2+ binding and phosphorylation of the active site residue Asp8. Initial velocity techniques were used to define the steady-state kinetic constants k(cat) = 177 +/- 9 s(-1), K-m = 49 +/- 4 mu
