Modifying Fletchervirus with CRISPRi in Campylobacter jejuni and using PCR to create a synthetic genome
Campylobacter jejuni causes around 100 million cases of gastroenteritis each year and are often transmitted to humans through the consumption of contaminated food, especially poultry. One of the natural enemies of C. jejuni is the bacteriophage Fletchervirus. In this master thesis I have been working on developing and evaluating different tools to modify Fletchervirus which could in the future hel