Translocation of STIM1 and STIM2 from the endoplasmic reticulum to the plasma membrane is a key step in store-operated calcium entry in the cell. We show by isothermal titration calorimetry that calmodulin binds in a calcium-dependent manner to the polybasic C-termini of STIM1 and STIM2, a region critical for their translocation to the plasma membrane ( K D

During formation of polymorphonuclear neutrophils, proteins are synthesized for storage in granules. Whereas sorting of proteins into distinct subtypes of cytoplasmic granules may reflect the coordinated expression of the proteins contained in them, still the mechanism(s) for the retrieval of proteins from the constitutive secretion is unknown. To investigate the mechanisms of retrieval, nonmyeloi

A natural anticoagulant pathway denoted the protein C system provides specific and efficient control of blood coagulation. Protein C is the key component of the system and circulates in the blood as a zymogen to an anticoagulant serine protease.Activation of protein C is achieved on the surface of endothelial cells by thrombin bound to the membrane protein thrombomodulin. The endothelial protein C

Although dic(9;20)(p13.2;q11.2) is a characteristic abnormality in childhood B-cell precursor acute lymphoblastic leukemias (BCP ALL), little is known about its clinical impact or the type and frequency of additional aberrations it may occur together with. We here review the clinical and cytogenetic features of a Nordic pediatric series of 24 patients with dic(9;20)-positive BCP ALL diagnosed 1996

Although there is abundant evidence that hyperglucagonaemia plays a key role in the development of hyperglycaemia in type 2 diabetes, efforts to understand and correct this abnormality have been overshadowed by the emphasis on insulin secretion and action. However, recognition that the incretin hormone glucagon-like peptide-1 (GLP-1) exerts opposing effects on glucagon and insulin secretion has re

In our continuing research on cytotoxic components from the Formosan pteridophyte Thelypteris torresiana (GAUD.) ALSTONONE, two new compounds, a novel flavonoid, flavotorresin (1), and a flavonoid diglycoside, multi-florin C (2), along with five known compounds, were isolated. The structural elucidation was established on the basis of spectroscopic data analysis. The possible biosynthetic pathway

In this study, a part of an automotive side front section (front side member inner) was studied and a comparison both regarding material behaviour and of accuracy of the FE simulations was made. Mild steel, Rephos steel and TRIP700 were compared both experimentally and numerically. The results showed that TRIP steel has a significantly larger springback than the other materials. Furthermore, the F

Using exact dispersion relations for electromagnetic wave propagation in layered, periodic media, consisting of two phases, we derive explicit asymptotic solutions for small wavenumbers. These solutions are compared to the numerical solutions of the exact dispersion relations, and applications to homogenization problems are discussed. The results can be used as test cases for homogenization techni

Purpose – This paper seeks to investigate the role of leadership styles in the implementation of evidence-based treatment methods (EBP) for drug abuse and criminal behaviour. Design/methodology/approach – The paper employs a triangulation approach through mail questionnaires to 112 treatment personnel (49 per cent response rate), interviews with 65 employees and managers, observations and feedbac

The first part of this study concerns the aqueous phase behavior of mixtures of diglycerol monooleate (DGMO) and glycerol dioleate (GDO) examined by X-ray diffraction (XRD). The ternary phase diagram displays a multitude of liquid crystalline phases (polymorphism). With increasing GDO content the following phase sequence was observed: lamellar (L-alpha); two reversed bicontinuous cubic phases (Q(2

Mutagenic/carcinogenic heterocyclic amines (HAs) are formed in the crust during the cooking of meat. The influence of cooking loss, time, and temperature on the formation of HAs was investigated in fried beefburgers. 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), and 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx) were

Results of experiments are compared to simulations of the performance of V2O5/gamma-alumina SCR catalyst used to remove nitrogen oxides from diesel exhausts. The kinetic model is based on Eley-Rideal kinetics. Temperature programmed desorption (TPD) of the ammonia is used to evaluate adsorption/desorption parameters. Stationary experiments on a 3.6-dm(3) engine with a 4.8-dm(3) honeycomb catalyst

The endo-beta-1,4-mannanase encoding gene man1 of Aspergillus aculeatus MRC11624 was amplified from mRNA by polymerase chain reaction using sequence-specific primers designed from the published sequence of man1 from A. aculeatus KSM510. The amplified fragment was cloned and expressed in Saccharomyces cerevisiae under the gene regulation of the alcohol dehydrogenase ( ADH2PT ) and phosphoglycerate

Photovoltaic modules in two-dimensional low-concentrating systems with specular parabolic reflectors often experience high local irradiance that causes high local currents and cell temperatures. This generally results in power losses. The use of low-angle scattering reflectors gives a smoother irradiance distribution, which results in a higher fill factor. In order to study how the choice of refle

Objectives. This study aimed at identifying the genetic events involved in immortalization of ovarian epithelial cells, which might be important steps in ovarian carcinogenesis. Methods. The genetic profiles of five human ovarian surface epithelial (HOSE) cell lines immortalized by retroviral transfection of the human papillomavirus (HPV) E6/E7 genes were thoroughly characterized by chromosome ban

The study examines globalization processes in property markets through an empirical investigation into the commercial property market of Greater Copenhagen. The focus is on investment in commercial properties. Globalization of property markets is defined, a framework of analysis presented and methodological problems reported. The analysis reveals a marked increase in the volumes and shares of fore

The ultimate goal in the treatment of haemophilia patients with inhibitors is to eradicate permanently the inhibitor and induce tolerance. Here we summarize our experience at the Malmo centre regarding tolerance induction according to the Malmo Treatment Model. The protocol includes immunoadsorption if needed, neutralization of inhibitor and replacement with factor concentrates, cyclophosphamide i

The RUNX1 gene encodes the alpha subunit of the core binding factor (CBF) and is a common target of genetic mutations in acute leukemia. We propose that RUNX1 is a gatekeeper gene, the disruption of which leads to the exodus of a subset of hematopoietic progenitors with increased self-renewal potential from the normal environmental controls of homeostasis. This pool of "escaped" cells is the targe

The runoff from real 3 cm thick sedum-moss roofs and from laboratory roof plots in southern Sweden is measured and analysed. Real rains and artificial storms are used for the analysis. The probability of high runoff is compared with the probability of high precipitation intensity. Intensity-duration-frequency curves for runoff are derived and it is found that the runoff of 1.5 year return period c